Chen Chuchu, Fu Yonghao, Sparks Sonja S, Lyu Zhaoyuan, Pradhan Arijit, Ding Shichao, Boddeti Narasimha, Liu Yun, Lin Yuehe, Du Dan, Qiu Kaiyan
School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States.
Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
ACS Sens. 2024 Jun 28;9(6):3212-3223. doi: 10.1021/acssensors.4c00528. Epub 2024 May 31.
Wearable sweat biosensors have shown great progress in noninvasive, , and continuous health monitoring to demonstrate individuals' physiological states. Advances in novel nanomaterials and fabrication methods promise to usher in a new era of wearable biosensors. Here, we introduce a three-dimensional (3D)-printed flexible wearable health monitor fabricated through a unique one-step continuous manufacturing process with self-supporting microfluidic channels and novel single-atom catalyst-based bioassays for measuring the sweat rate and concentration of three biomarkers. Direct ink writing is adapted to print the microfluidic device with self-supporting structures to harvest human sweat, which eliminates the need for removing sacrificial supporting materials and addresses the contamination and sweat evaporation issues associated with traditional sampling methods. Additionally, the pick-and-place strategy is employed during the printing process to accurately integrate the bioassays, improving manufacturing efficiency. A single-atom catalyst is developed and utilized in colorimetric bioassays to improve sensitivity and accuracy. A feasibility study on human skin successfully demonstrates the functionality and reliability of our health monitor, generating reliable and quantitative results of sweat rate, glucose, lactate, and uric acid concentrations during physical exercise.
可穿戴汗液生物传感器在无创、连续的健康监测以展示个体生理状态方面已取得巨大进展。新型纳米材料和制造方法的进步有望开创可穿戴生物传感器的新时代。在此，我们介绍一种通过独特的一步连续制造工艺制造的三维（3D）打印柔性可穿戴健康监测器，其具有自支撑微流体通道和基于新型单原子催化剂的生物测定法，用于测量汗液速率和三种生物标志物的浓度。采用直接墨水书写来打印具有自支撑结构的微流体装置以收集人体汗液，这消除了去除牺牲支撑材料的需要，并解决了与传统采样方法相关的污染和汗液蒸发问题。此外，在打印过程中采用拾取和放置策略来精确整合生物测定法，提高制造效率。开发了一种单原子催化剂并将其用于比色生物测定法以提高灵敏度和准确性。对人体皮肤的可行性研究成功证明了我们健康监测器的功能和可靠性，在体育锻炼期间生成了汗液速率、葡萄糖、乳酸和尿酸浓度的可靠定量结果。
