College of Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China.
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Biomater Adv. 2022 Mar;134:112549. doi: 10.1016/j.msec.2021.112549. Epub 2021 Nov 16.
The preparation of bioactive materials with biomolecules as templates to control the nucleation and growth of nano-hydroxyapatite (n-HA) crystals is a vital research field in bone tissue engineering. However, meeting the performance requirements of possessing appropriate surface roughness, high porosity, structural stability, adequate mechanical strength, biodegradability and biocompatibility at the same time is the core issue that restricts the development of these biomimetic materials in biosciences as well as medical clinical translation. In this work, a mineralized self-assembled silk fibroin (SF)/cellulose interpenetrating network composite aerogel (M-S-C) material was prepared by freeze-drying using sol-gel and in situ mineralization strategy. The effects of the main factors, such as the surface properties of SF macromolecules and the change of mineralization time, on the n-HA self-assembly process and the property of M-S-C under defined conditions were explored. The properties of M-S-C, including the physicochemical properties, morphology, mechanical property, degradation behavior and in vitro cytotoxicity, were investigated to evaluate its application prospects in bone tissue engineering. M-S-C exhibits the microstructure required for an ideal cancellous bone repair material, porosity up to 99.2%, high thermal stability, moderately adjustable compressive strength (12.7-22.4 MPa), and appreciable in vitro degradation rate. Moreover, M-S-C extracts can significantly accelerate the proliferation of human embryonic kidney cells. This mineralized interpenetrating polymer network aerogel material with excellent comprehensive performance shows potential for application in bone repair and regeneration.
以生物分子为模板来制备具有生物活性的材料，以控制纳米羟基磷灰石(n-HA)晶体的成核和生长，这是骨组织工程中一个重要的研究领域。然而，同时满足具有适当表面粗糙度、高孔隙率、结构稳定性、足够机械强度、可生物降解性和生物相容性的性能要求，是限制这些仿生材料在生物科学以及医学临床转化中发展的核心问题。在这项工作中，采用溶胶-凝胶和原位矿化策略，通过冷冻干燥制备了矿化自组装丝素蛋白(SF)/纤维素互穿网络复合水凝胶(M-S-C)材料。探讨了 SF 大分子表面性质和矿化时间等主要因素对 n-HA 自组装过程和在规定条件下 M-S-C 性能的影响。对 M-S-C 的性能，包括物理化学性能、形貌、力学性能、降解行为和体外细胞毒性进行了研究，以评估其在骨组织工程中的应用前景。M-S-C 具有理想的松质骨修复材料所需的微观结构，孔隙率高达 99.2%，热稳定性高，压缩强度可适度调节(12.7-22.4 MPa)，体外降解速率也较高。此外，M-S-C 浸提液能显著促进人胚肾细胞的增殖。这种具有优异综合性能的矿化互穿聚合物网络水凝胶材料在骨修复和再生方面具有应用潜力。
