Department of Surgery, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Surg Infect (Larchmt). 2022 Jun;23(5):451-457. doi: 10.1089/sur.2022.022. Epub 2022 May 6.
Cystic echinococcosis (CE) is caused by . In Central Iran, no molecular information is available on CE in humans. Therefore, in this study, we identified the genotyping of hydatid cysts obtained from patients with CE in central Iran using mitochondrial cytochrome c oxidase subunit I (1) gene. Hydatid cysts were obtained from 19 patients referred to Shahid Sadoughi, Mojibian, and Mortaz Hospitals, Yazd, Iran from 2018 to 2020. Informed consent was obtained from all included patients. After DNA extraction, amplification was done using 1 gene. Phylogenetic analysis was performed using MEGA7. Of the 19 patients, 11 (57.9%) were male and eight (42.1%) were female. The mean age of the patients was 35.645 ± 2.55 years old. Regarding cyst location, of eight isolates from lung, six and two belonged to G1 and G6, respectively; and all liver cysts were G1 genotype. The spleen and neck cysts had G1 and G6 genotypes, respectively (p > 0.05). All cysts with a diameter in the range of 5-10 cm (n = 9) and large cysts (>10 cm; n = 5) were identified as G1 (p = 0.002). The maximum likelihood tree topology demonstrated the maximum similarity of G1 among Iran and worldwide (99%-100% likelihood). Based on our results, it seems that the sheep-dog cycle in the infection of humans by in this study area has the most important role compared with the other cycles such as the camel-dog one.
囊性包虫病（CE）是由 引起的。在伊朗中部，尚无关于人类 CE 的分子信息。因此，在本研究中，我们使用线粒体细胞色素 c 氧化酶亚基 I（1）基因鉴定了来自伊朗中部患有 CE 的患者的包虫囊肿的基因分型。 从 2018 年至 2020 年，从伊朗亚兹德的沙希德萨多希、莫吉比安和莫尔塔扎医院获得了 19 名患者的包虫囊肿。所有纳入的患者均获得了知情同意。提取 DNA 后，使用 1 基因进行扩增。使用 MEGA7 进行系统发育分析。 在 19 名患者中，11 名（57.9%）为男性，8 名（42.1%）为女性。患者的平均年龄为 35.645 ± 2.55 岁。关于囊肿位置，来自肺的 8 个分离物中，6 个和 2 个分别属于 G1 和 G6；所有肝囊肿均为 G1 基因型。脾和颈部囊肿分别具有 G1 和 G6 基因型（p>0.05）。所有直径在 5-10cm 范围内的囊肿（n=9）和大囊肿（>10cm；n=5）均被鉴定为 G1（p=0.002）。最大似然树拓扑显示，G1 在伊朗和全球范围内的相似度最高（99%-100%可能性）。 根据我们的结果，与其他循环（如骆驼-犬循环）相比，在本研究区域中，羊-犬循环在人类感染中的作用似乎最为重要。
