Chen Jun, Du Yichun, Zhu Wei, Pang Xin, Wang Zhen
Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, People's Republic of China.
Faculty of Horticultural Science and Technology, Suzhou 215008, People's Republic of China.
Open Life Sci. 2022 Apr 22;17(1):381-392. doi: 10.1515/biol-2022-0048. eCollection 2022.
Long-term fertilization will affect the above-ground vegetation, but we have little understanding of soil bacterial community structure and diversity so far. This study aims to study the effect of organic fertilization on the soil bacterial community structure and diversity of protected long-term continuous tomato cropping by using high-throughput sequencing technology. Results show that (1) fertilization application (chemical fertilizer [CF] and vermicompost [VM]) significantly changed the soil physico-chemistry properties, such as soil pH decreased compared with control treatment and increased the soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), and total potassium (TK) contents; (2) VM increased the Shannon index of soil bacteria but decreased the soil Chao1 index; and (3) soil Proteobacteria and Actinomycetes were dominant taxa and the relative abundance of Actinobacteria increased by 36.40-44.27 and 25.80-29.35%, with CF and VM, respectively, compared with the control. Pearson correlation analysis showed that soil pH, SOC, TN, TP, and TK were the main environmental factors that affected the diversity and richness of soil bacterial communities. Redundancy analysis (RDA) showed that the SOC and TN play important roles in the composition of soil bacterial communities. In summary, the effect of VM on the soil bacterial community structure of continuous tomato cropping is better than that of CF, which should be used in the sustainable production of facility tomatoes.
长期施肥会影响地上植被，但目前我们对土壤细菌群落结构和多样性了解甚少。本研究旨在利用高通量测序技术研究有机肥对长期连作保护地番茄土壤细菌群落结构和多样性的影响。结果表明：（1）施肥处理（化肥[CF]和蚯蚓粪[VM]）显著改变了土壤理化性质，如与对照处理相比土壤pH降低，土壤有机碳（SOC）、全氮（TN）、全磷（TP）和全钾（TK）含量增加；（2）VM提高了土壤细菌的香农指数，但降低了土壤Chao1指数；（3）土壤变形菌门和放线菌门是优势类群，与对照相比，CF和VM处理下放线菌门的相对丰度分别增加了36.40 - 44.27%和25.80 - 29.35%。Pearson相关性分析表明，土壤pH、SOC、TN、TP和TK是影响土壤细菌群落多样性和丰富度的主要环境因素。冗余分析（RDA）表明，SOC和TN在土壤细菌群落组成中起重要作用。综上所述，VM对连作番茄土壤细菌群落结构的影响优于CF，应在设施番茄的可持续生产中应用。
