Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, School of Landscape Architecture, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China.
PeerJ. 2022 May 3;10:e13273. doi: 10.7717/peerj.13273. eCollection 2022.
The Sugars Will Eventually be Exported Transporter (SWEET) gene family encodes a family of sugar transporters that play essential roles in plant growth, reproduction, and biotic and abiotic stresses. is a considerable ornamental wood plant with high edible and medicinal values; however, its lack of tolerance to low temperature has severely limited its geographical distribution. To investigate whether this gene family mediates the response of to cold stress, we identified that the gene family consists of 17 members and divided the family members into four groups. Sixteen of these genes were anchored on six chromosomes, and one gene was anchored on the scaffold with four pairs of segmental gene duplications and two pairs of tandem gene duplications. -acting regulatory element analysis indicated that the genes are potentially involved in development, including potentially regulating roles in procedure, such as circadian control, abscisic acid-response and light-response, and responses to numerous stresses, such as low-temperature and drought. We performed low-temperature treatment in the cold-tolerant cultivar 'Songchun' and cold-sensitive cultivar 'Zaolve' and found that the expression of four of 17 was either upregulated or downregulated with prolonged treatment times. This finding indicates that these family members may potentially play a role in cold stress responses in . Our study provides a basis for further investigation of the role of SWEET proteins in the development of and its responses to cold stress.
糖最终将被输出转运蛋白（SWEET）基因家族编码了一类糖转运蛋白，它们在植物生长、繁殖以及生物和非生物胁迫中起着至关重要的作用。是一种具有很高食用和药用价值的可观的观赏木本植物；然而，它对低温的耐受性差严重限制了其地理分布。为了研究这个基因家族是否介导了对低温胁迫的响应，我们鉴定出 基因家族由 17 个成员组成，并将家族成员分为四个组。这 16 个基因被锚定在六个染色体上，一个基因被锚定在支架上，有四对片段基因重复和两对串联基因重复。- 作用调控元件分析表明，这些 基因可能参与 发育，包括潜在地调节生物钟控制、脱落酸响应和光响应等过程，以及对低温和干旱等多种胁迫的响应。我们在耐寒品种“松春”和敏感品种“枣乐”中进行了低温处理，发现随着处理时间的延长，17 个 中的 4 个的表达要么上调，要么下调。这一发现表明，这些家族成员可能在 对低温胁迫的响应中发挥作用。我们的研究为进一步研究 SWEET 蛋白在 发育及其对低温胁迫响应中的作用提供了基础。
