Choi Su-Young, Lee Su Yeon, Kim Hyung Gyun, Jeong Jae Cheon, Batara Don Carlo, Kim Sung-Hak, Cho Jeong-Yong
Department of Animal Science, Chonnam National University, 77 Yongbongro, Gwangju, 61186 Republic of Korea.
Department of Food Science and Technology, Chonnam National University, 77 Yongbongro, Gwangju, 61186 Republic of Korea.
Food Sci Biotechnol. 2022 Mar 28;31(5):617-625. doi: 10.1007/s10068-022-01055-6. eCollection 2022 May.
Mycosporine-like amino acids (MAAs) such as shinorine and porphyra-334 from spp. are bioactive compounds with strong photoprotective and antioxidant properties. In this study, the anti-adipogenic effect of shinorine and porphyra-334 was examined in vitro utilizing 3T3-L1 preadipocytes. Shinorine and porphyra-334 were extracted from laver () 50% methanolic (MeOH) extract of and their structures were elucidated by MS and NMR spectroscopy. Both compounds had no cytotoxic effect in 3T3-L1 cells (< 200 μg/mL) and inhibited the accumulation of lipid droplets in 3T3-L1 mature adipocytes in a dose-dependent manner (0.1 and 1.0 μM). Interestingly, both compounds had also significantly reduced the expression of adipogenic-related genes such as peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer-binding protein α (C/EBPα), adiponectin, and leptin in 3T3-L1 cells. The findings suggest that shinorine and porphyra-334 have the potential to inhibit adipogenesis in 3T3-L1 preadipocytes.
来自紫菜属物种的诸如紫菜素和紫菜-334等类菌孢素氨基酸（MAAs）是具有强大光保护和抗氧化特性的生物活性化合物。在本研究中，利用3T3-L1前脂肪细胞在体外检测了紫菜素和紫菜-334的抗脂肪生成作用。紫菜素和紫菜-334从紫菜的50%甲醇提取物中提取，并通过质谱和核磁共振光谱对其结构进行了阐明。这两种化合物在3T3-L1细胞中（<200μg/mL）均无细胞毒性作用，并以剂量依赖性方式（0.1和1.0μM）抑制3T3-L1成熟脂肪细胞中脂滴的积累。有趣的是，这两种化合物还显著降低了3T3-L1细胞中脂肪生成相关基因如过氧化物酶体增殖物激活受体γ2（PPARγ2）、CCAAT/增强子结合蛋白α（C/EBPα）、脂联素和瘦素的表达。这些发现表明，紫菜素和紫菜-334有潜力抑制3T3-L1前脂肪细胞的脂肪生成。
