• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

提取物对3T3-L1脂肪细胞分化的抑制作用。

Inhibitory effects of extract on 3T3-L1 adipocyte differentiation.

作者信息

Choi Su-Young, Lee Su Yeon, Jang Da Hye, Lee Suk Jun, Cho Jeong-Yong, Kim Sung-Hak

机构信息

Department of Animal Science, Chonnam National University, Gwangju 61186, Korea.

Department of Food Science and Technology, Chonnam National University, Gwangju 61186, Korea.

出版信息

J Anim Sci Technol. 2020 Nov;62(6):854-863. doi: 10.5187/jast.2020.62.6.854. Epub 2020 Nov 30.

DOI:10.5187/jast.2020.62.6.854
PMID:33987565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7721580/
Abstract

This study was aimed to investigate the inhibitory effects of () extract on the adipogenesis of 3T3-L1 cells and evaluate its anti-obesity effect. The proliferation of 3T3-L1 cells and differentiation of adipocytes under treatment of extract was examined by measuring the cell viability using alamarBlue assay and lipid droplets by Oil Red O staining. Results showed that extract has no cytotoxicity effect and lipid droplets formation decreased in a concentration-dependent manner in 3T3-L1 cells. It has been confirmed that transcription factors affecting lipid accumulation and anti-adipogenic effects during cell differentiation are linked to extract. We observed that shows lowering the mRNA expression of peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer binding protein α (C/EBPα) that adipogenesis-associated key transcription factors and inhibiting adipogenesis in the early stages of differentiation. Treating the cells with did not only suppressed PPARγ2 and C/EBPα but also significantly decreased the mRNA expression of adiponectin, Leptin, fatty acid synthase, adipocyte protein 2, and Acetyl-coA carboxylase 1. Overall, the extract demonstrated inhibitory property in adipogenesis, which has a potential effect in anti-obesity in 3T3-L1 cells.

摘要

本研究旨在探讨（）提取物对3T3-L1细胞脂肪生成的抑制作用，并评估其抗肥胖效果。通过alamarBlue法测量细胞活力以及用油红O染色检测脂滴，研究提取物处理下3T3-L1细胞的增殖和脂肪细胞分化情况。结果表明，提取物无细胞毒性作用，且3T3-L1细胞中脂滴形成呈浓度依赖性减少。已证实，影响细胞分化过程中脂质积累和抗脂肪生成作用的转录因子与提取物有关。我们观察到，提取物可降低过氧化物酶体增殖物激活受体γ2（PPARγ2）、CCAAT/增强子结合蛋白α（C/EBPα）的mRNA表达，这两种蛋白是脂肪生成相关的关键转录因子，并在分化早期抑制脂肪生成。用提取物处理细胞不仅抑制了PPARγ2和C/EBPα，还显著降低了脂联素、瘦素、脂肪酸合酶、脂肪细胞蛋白2和乙酰辅酶A羧化酶1的mRNA表达。总体而言，提取物在脂肪生成中表现出抑制特性，对3T3-L1细胞的抗肥胖具有潜在作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/d97416fb0992/jast-62-6-854-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/cc245959a4d6/jast-62-6-854-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/3eacd9cfe9b1/jast-62-6-854-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/d97416fb0992/jast-62-6-854-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/cc245959a4d6/jast-62-6-854-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/3eacd9cfe9b1/jast-62-6-854-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a615/7721580/d97416fb0992/jast-62-6-854-g3.jpg

相似文献

1
Inhibitory effects of extract on 3T3-L1 adipocyte differentiation.提取物对3T3-L1脂肪细胞分化的抑制作用。
J Anim Sci Technol. 2020 Nov;62(6):854-863. doi: 10.5187/jast.2020.62.6.854. Epub 2020 Nov 30.
2
Shinorine and porphyra-334 isolated from laver () inhibit adipogenesis in 3T3-L1 cells.从紫菜中分离出的紫菜红素和紫菜-334抑制3T3-L1细胞的脂肪生成。
Food Sci Biotechnol. 2022 Mar 28;31(5):617-625. doi: 10.1007/s10068-022-01055-6. eCollection 2022 May.
3
Suppression of adipocyte differentiation and lipid accumulation by stearidonic acid (SDA) in 3T3-L1 cells.硬脂烯酸（SDA）抑制 3T3-L1 细胞中的脂肪细胞分化和脂质积累。
Lipids Health Dis. 2017 Sep 25;16(1):181. doi: 10.1186/s12944-017-0574-7.
4
α, β-Amyrin, a pentacyclic triterpenoid from Protium heptaphyllum suppresses adipocyte differentiation accompanied by down regulation of PPARγ and C/EBPα in 3T3-L1 cells.从坡垒中提取的五环三萜 α, β-齐墩果酸通过下调 3T3-L1 细胞中过氧化物酶体增殖物激活受体 γ 和 CCAAT/增强子结合蛋白 α 的表达抑制脂肪细胞分化。
Biomed Pharmacother. 2019 Jan;109:1860-1866. doi: 10.1016/j.biopha.2018.11.027. Epub 2018 Nov 26.
5
Korean Chungtaejeon tea extract attenuates body weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes.韩国忠太煎茶提取物可减轻 C57BL/6J-Lep ob/ob 小鼠体重增加，并调节 3T3-L1 脂肪细胞的脂肪生成和脂肪分解。
J Integr Med. 2017 Jan;15(1):56-63. doi: 10.1016/S2095-4964(17)60321-2.
6
Inhibitory effect of ethanolic extract of leaf on 3T3-L1 adipocyte differentiation.叶乙醇提取物对3T3-L1脂肪细胞分化的抑制作用。
Nutr Res Pract. 2021 Oct;15(5):555-567. doi: 10.4162/nrp.2021.15.5.555. Epub 2021 Mar 11.
7
extract (CQR-300) inhibits lipid accumulation by downregulating adipogenesis and lipogenesis in 3T3-L1 cells.提取物（CQR-300）通过下调3T3-L1细胞中的脂肪生成和脂质生成来抑制脂质积累。
Toxicol Rep. 2018 Mar 30;5:608-614. doi: 10.1016/j.toxrep.2018.02.008. eCollection 2018.
8
Platyphylloside Isolated From Inhibit Adipocyte Differentiation and Induce Lipolysis Via Regulating Adipokines Including PPARγ in 3T3-L1 Cells.从……中分离出的桔梗皂苷通过调节3T3-L1细胞中包括PPARγ在内的脂肪因子来抑制脂肪细胞分化并诱导脂肪分解。
Pharmacogn Mag. 2016 Oct-Dec;12(48):276-281. doi: 10.4103/0973-1296.192208.
9
Glycine enhances expression of adiponectin and IL-10 in 3T3-L1 adipocytes without affecting adipogenesis and lipolysis.甘氨酸可增强 3T3-L1 脂肪细胞中脂联素和白细胞介素-10 的表达，而不影响脂肪生成和脂肪分解。
Amino Acids. 2018 May;50(5):629-640. doi: 10.1007/s00726-018-2537-3. Epub 2018 Jan 22.
10
The role and possible mechanism of lncRNA U90926 in modulating 3T3-L1 preadipocyte differentiation.长链非编码RNA U90926在调节3T3-L1前脂肪细胞分化中的作用及可能机制
Int J Obes (Lond). 2017 Feb;41(2):299-308. doi: 10.1038/ijo.2016.189. Epub 2016 Oct 26.

引用本文的文献

1
Dietary supplementation with probiotics promotes weight loss by reshaping the gut microbiome and energy metabolism in obese dogs.膳食补充益生菌可通过重塑肥胖犬的肠道微生物组和能量代谢来促进减肥。
Microbiol Spectr. 2024 Mar 5;12(3):e0255223. doi: 10.1128/spectrum.02552-23. Epub 2024 Jan 25.
2
Effects of Fermented L. and L. on Inhibition of Obesity In Vitro and In Mice.发酵的 L. 和 L. 对体外和小鼠肥胖抑制的影响。
Nutrients. 2023 Apr 22;15(9):2022. doi: 10.3390/nu15092022.
3
Association of Seaweed Consumption with Metabolic Syndrome and Its Components: Findings from the Korean Genome and Epidemiology Study.

本文引用的文献

1
Anti-Obesity Effects of Macroalgae.海藻的抗肥胖作用。
Nutrients. 2020 Aug 8;12(8):2378. doi: 10.3390/nu12082378.
2
Health Functionality and Quality Control of Laver (, ): Current Issues and Future Perspectives as an Edible Seaweed.紫菜（, ）的健康功能与质量控制：作为食用海藻的当前问题与未来展望。
Mar Drugs. 2019 Dec 23;18(1):14. doi: 10.3390/md18010014.
3
Protective effect of MAAs extracted from Porphyra tenera against UV irradiation-induced photoaging in mouse skin.从紫菜中提取的 MAAs 对 UV 辐射诱导的小鼠皮肤光老化的保护作用。
海藻消费与代谢综合征及其组分的关联：韩国基因组与流行病学研究的结果
Foods. 2022 Jun 1;11(11):1635. doi: 10.3390/foods11111635.
4
Shinorine and porphyra-334 isolated from laver () inhibit adipogenesis in 3T3-L1 cells.从紫菜中分离出的紫菜红素和紫菜-334抑制3T3-L1细胞的脂肪生成。
Food Sci Biotechnol. 2022 Mar 28;31(5):617-625. doi: 10.1007/s10068-022-01055-6. eCollection 2022 May.
5
Chebulinic Acid Suppresses Adipogenesis in 3T3-L1 Preadipocytes by Inhibiting PPP1CB Activity.鞣花酸通过抑制 PPP1CB 活性抑制 3T3-L1 前脂肪细胞的脂肪生成。
Int J Mol Sci. 2022 Jan 13;23(2):865. doi: 10.3390/ijms23020865.
J Photochem Photobiol B. 2019 Mar;192:26-33. doi: 10.1016/j.jphotobiol.2018.12.009. Epub 2018 Dec 19.
4
Health Benefits and Pharmacological Effects of Porphyra Species.紫菜属的健康益处和药理作用。
Plant Foods Hum Nutr. 2019 Mar;74(1):10-17. doi: 10.1007/s11130-018-0707-9.
5
LY3009120, a pan-Raf kinase inhibitor, inhibits adipogenesis of 3T3-L1 cells by controlling the expression and phosphorylation of C/EBP-α, PPAR-γ, STAT‑3, FAS, ACC, perilipin A, and AMPK.LY3009120，一种泛 RAF 激酶抑制剂，通过控制 C/EBP-α、PPAR-γ、STAT-3、FAS、ACC、 perilipin A 和 AMPK 的表达和磷酸化来抑制 3T3-L1 细胞的脂肪生成。
Int J Mol Med. 2018 Dec;42(6):3477-3484. doi: 10.3892/ijmm.2018.3890. Epub 2018 Sep 21.
6
Impaired Adipogenesis and Dysfunctional Adipose Tissue in Human Hypertrophic Obesity.人类肥胖症中的脂肪生成受损和脂肪组织功能障碍。
Physiol Rev. 2018 Oct 1;98(4):1911-1941. doi: 10.1152/physrev.00034.2017.
7
Adiponectin Regulation and Function.脂联素的调节与功能
Compr Physiol. 2018 Jun 18;8(3):1031-1063. doi: 10.1002/cphy.c170046.
8
Increase in anti-inflammatory activities of radical-degraded porphyrans isolated from discolored nori (Pyropia yezoensis).褪色紫菜（Pyropia yezoensis）中原卟啉自由基降解产物抗炎活性的增加。
Int J Biol Macromol. 2018 Oct 1;117:78-86. doi: 10.1016/j.ijbiomac.2018.05.146. Epub 2018 May 22.
9
Inhibitory effect of sulphated polysaccharide porphyran (isolated from Porphyra yezoensis) on RANKL-induced differentiation of RAW264.7 cells into osteoclasts.硫酸化多糖岩藻依聚糖（从紫菜中分离得到）对 RANKL 诱导 RAW264.7 细胞向破骨细胞分化的抑制作用。
Phytother Res. 2018 Mar;32(3):452-458. doi: 10.1002/ptr.5988. Epub 2017 Dec 11.
10
Phenolic compounds as natural and multifunctional anti-obesity agents: A review.酚类化合物作为天然多功能抗肥胖剂：综述。
Crit Rev Food Sci Nutr. 2019;59(8):1212-1229. doi: 10.1080/10408398.2017.1399859. Epub 2017 Nov 20.