Song Meijun, Zhu Haidong, Ye Lin, Liu Chengxiang, Xu Zhaojun
Department of Respiratory Medicine, Zhejiang Provincial People's Hospital Hangzhou China 310016.
Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University Hangzhou China 310016.
RSC Adv. 2019 Oct 14;9(56):32683-32690. doi: 10.1039/c9ra03365c. eCollection 2019 Oct 10.
Biomaterial-associated infections (BAIs) remain a major challenge in clinical surgery because they can potentially cause serious disabilities in patients. This study investigated the use of a multilayer coating technology that can co-deliver two therapeutic components, gentamicin and OP-145 peptide, to treat effectively. A biocompatible and biodegradable thin film was produced layer-by-layer assembly using an antibacterial peptide and gentamicin. The thin film was systematically characterized, showing controllable features such as thickness, transparency, cargo loading, and cargo release. tests showed that the thin film has fewer toxicity problems than soluble cargos; compared to cargos in a soluble form, the thin film has minor impacts on mammalian cells' metabolism. Additionally, the antibacterial cargos assembled on the thin film can effectively inhibit the formation of biofilms and show effective antibacterial potency. studies illustrated that the thin film can inhibit the progression of in a mouse model, indicating the effectiveness of the thin film structure in the clinic. This study demonstrates that a thin film composed of gentamicin and OP-145 could be employed to prevent BAIs in clinical surgery.
生物材料相关感染（BAIs）仍是临床手术中的一项重大挑战，因为它们可能会给患者带来严重残疾。本研究调查了一种多层涂层技术的应用，该技术能够共同递送两种治疗成分——庆大霉素和OP-145肽，以实现有效治疗。通过使用抗菌肽和庆大霉素进行逐层组装，制备出了一种生物相容性和可生物降解的薄膜。对该薄膜进行了系统表征，结果显示其具有诸如厚度、透明度、载药量和药物释放等可控特性。测试表明，该薄膜相比可溶性药物具有更少的毒性问题；与可溶性形式的药物相比，该薄膜对哺乳动物细胞代谢的影响较小。此外，组装在薄膜上的抗菌药物能够有效抑制生物膜的形成，并显示出有效的抗菌效力。体内研究表明，该薄膜能够在小鼠模型中抑制感染的进展，这表明该薄膜结构在临床上具有有效性。本研究证明，由庆大霉素和OP-145组成的薄膜可用于预防临床手术中的生物材料相关感染。
