Yang Xufang, Dong Jianjiang, Hao Yankun, Qi Yucheng, Liang Jun, Yan Lei, Wang Wenting
Department of Pathophysiology, Mudanjiang Medical University, Mudanjiang 157011, China.
Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, China.
Evid Based Complement Alternat Med. 2022 Apr 29;2022:3126094. doi: 10.1155/2022/3126094. eCollection 2022.
Osteoporosis is an age-related systemic bone disease that places a heavy burden on patients and society. In this study, we aimed to investigate the effects of naringin (NAR) on the osteogenic differentiation of human adipose-derived stromal cells (ADSCs). The results demonstrated that NAR pretreatment effectively abated HO-induced cell death and ROS accumulation in ADSCs undergoing osteogenic differentiation (ADSCs-OD). In addition, we also observed that the impaired extracellular matrix mineralization and ALP activity in HO-stimulated ADSCs-OD were notably rescued by NAR pretreatment. Moreover, the effects of HO exposure on Wnt/-catenin signaling in ADSCs-OD were largely reversed by NAR pretreatment. Collectively, our findings indicated that NAR could protect ADSCs-OD against HO-inhibited osteogenic differentiation.
骨质疏松症是一种与年龄相关的全身性骨病，给患者和社会带来沉重负担。在本研究中，我们旨在探讨柚皮苷（NAR）对人脂肪来源的基质细胞（ADSCs）成骨分化的影响。结果表明，NAR预处理有效减轻了HO诱导的成骨分化ADSCs（ADSCs-OD）的细胞死亡和ROS积累。此外，我们还观察到，NAR预处理显著挽救了HO刺激的ADSCs-OD中受损的细胞外基质矿化和ALP活性。此外，NAR预处理在很大程度上逆转了HO暴露对ADSCs-OD中Wnt/β-连环蛋白信号通路的影响。总的来说，我们的研究结果表明，NAR可以保护ADSCs-OD免受HO抑制的成骨分化。
