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液态超极化方法与核磁共振仪器的接口

Interfacing Liquid State Hyperpolarization Methods with NMR Instrumentation.

作者信息

Pham Pierce, Mandal Ratnamala, Qi Chang, Hilty Christian

机构信息

Department of Chemistry, Texas A&M University, 3255 TAMU, College Station, TX 77843, USA.

出版信息

J Magn Reson Open. 2022 Jun;10-11. doi: 10.1016/j.jmro.2022.100052. Epub 2022 Mar 10.

Abstract

Advances in liquid state hyperpolarization methods have enabled new applications of high-resolution NMR spectroscopy. Utilizing strong signal enhancements from hyperpolarization allows performing NMR spectroscopy at low concentration, or with high time resolution. Making use of the high, but rapidly decaying hyperpolarization in the liquid state requires new techniques to interface hyperpolarization equipment with liquid state NMR spectrometers. This article highlights rapid injection, high resolution NMR spectroscopy with hyperpolarization produced by the techniques of dissolution dynamic nuclear polarization (D-DNP) and para-hydrogen induced polarization (PHIP). These are popular, albeit not the only methods to produce high polarization levels for liquid samples. Gas and liquid driven sample injection techniques are compatible with both of these hyperpolarization methods. The rapid sample injection techniques are combined with adapted NMR experiments working in a single, or small number of scans. They expand the application of liquid state hyperpolarization to spins with comparably short relaxation times, provide enhanced control over sample conditions, and allow for mixing experiments to study reactions in real time.

摘要

液态超极化方法的进展使得高分辨率核磁共振光谱有了新的应用。利用超极化带来的强信号增强效应,可以在低浓度下或高时间分辨率下进行核磁共振光谱分析。利用液态中高但迅速衰减的超极化需要新技术,以便将超极化设备与液态核磁共振光谱仪连接起来。本文重点介绍了通过溶解动态核极化(D-DNP)和仲氢诱导极化(PHIP)技术产生的超极化进行快速注射、高分辨率核磁共振光谱分析。这些是为液体样品产生高极化水平的常用方法,尽管不是唯一的方法。气体和液体驱动的样品注射技术与这两种超极化方法都兼容。快速样品注射技术与适用于单次或少量扫描的核磁共振实验相结合。它们将液态超极化的应用扩展到具有相对较短弛豫时间的自旋体系,增强了对样品条件的控制,并允许进行混合实验以实时研究反应。

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