利用优化的碱基编辑器在水稻中实现高效的C到G编辑。 - Suppr

Efficient C-to-G editing in rice using an optimized base editor.

作者信息

Tian Yifu, Shen Rundong, Li Zuren, Yao Qi, Zhang Xuening, Zhong Dating, Tan Xinhang, Song Minglei, Han Han, Zhu Jian-Kang, Lu Yuming

机构信息

Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

Hunan Academy of Agricultural Sciences, Changsha, China.

出版信息

Plant Biotechnol J. 2022 Jul;20(7):1238-1240. doi: 10.1111/pbi.13841. Epub 2022 Jun 3.

DOI:10.1111/pbi.13841

PMID:35534986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9241366/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb3e/11382979/e3703168dc35/PBI-20-1238-g001.jpg

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Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems.

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Efficient C-to-G editing in rice using an optimized base editor.

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Int J Mol Sci. 2022 Jul 20;23(14):7990. doi: 10.3390/ijms23147990.

Gene Editing With TALEN and CRISPR/Cas in Rice.

Prog Mol Biol Transl Sci. 2017;149:81-98. doi: 10.1016/bs.pmbts.2017.04.006. Epub 2017 May 24.

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CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.

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Fusion of a rice endogenous -methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants.

aBIOTECH. 2024 Mar 21;5(2):127-139. doi: 10.1007/s42994-024-00138-8. eCollection 2024 Jun.

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本文引用的文献

Exploring C-To-G Base Editing in Rice, Tomato, and Poplar.

Front Genome Ed. 2021 Sep 15;3:756766. doi: 10.3389/fgeed.2021.756766. eCollection 2021.

Development of an efficient plant dual cytosine and adenine editor.

J Integr Plant Biol. 2021 Sep;63(9):1600-1605. doi: 10.1111/jipb.13146. Epub 2021 Aug 2.

Improved plant cytosine base editors with high editing activity, purity, and specificity.

Plant Biotechnol J. 2021 Oct;19(10):2052-2068. doi: 10.1111/pbi.13635. Epub 2021 Jun 7.

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.

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Trends Biotechnol. 2020 Mar;38(3):236-240. doi: 10.1016/j.tibtech.2019.08.001. Epub 2019 Aug 30.

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Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant.

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DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations.

Mol Plant. 2015 Sep;8(9):1431-3. doi: 10.1016/j.molp.2015.05.009. Epub 2015 May 30.

Efficient C-to-G editing in rice using an optimized base editor.

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