NASA Ames Research Center, Moffett Field, California, United States of America.
PLoS One. 2022 May 10;17(5):e0268253. doi: 10.1371/journal.pone.0268253. eCollection 2022.
Thermophiles and hyperthermophiles are those organisms which grow at high temperature (> 40°C). The unusual properties of these organisms have received interest in multiple fields of biological research, and have found applications in biotechnology, especially in industrial processes. However, there are few listings of thermophilic and hyperthermophilic organisms and their relevant environmental and physiological data. Such repositories can be used to standardize definitions of thermophile and hyperthermophile limits and tolerances and would mitigate the need for extracting organism data from diverse literature sources across multiple, sometimes loosely related, research fields. Therefore, we have developed ThermoBase, a web-based and freely available database which currently houses comprehensive descriptions for 1238 thermophilic or hyperthermophilic organisms. ThermoBase reports taxonomic, metabolic, environmental, experimental, and physiological information in addition to literature resources. This includes parameters such as coupling ions for chemiosmosis, optimal pH and range, optimal temperature and range, optimal pressure, and optimal salinity. The database interface allows for search features and sorting of parameters. As such, it is the goal of ThermoBase to facilitate and expedite hypothesis generation, literature research, and understanding relating to thermophiles and hyperthermophiles within the scientific community in an accessible and centralized repository. ThermoBase is freely available online at the Astrobiology Habitable Environments Database (AHED; https://ahed.nasa.gov), at the Database Center for Life Science (TogoDB; http://togodb.org/db/thermobase), and in the S1 File.
嗜热菌和超嗜热菌是指在高温（>40°C）下生长的生物体。这些生物体的特殊性质引起了多个生物研究领域的关注，并在生物技术中找到了应用，特别是在工业过程中。然而，目前有关嗜热菌和超嗜热菌的生物体及其相关环境和生理数据的列表很少。这样的存储库可用于标准化嗜热菌和超嗜热菌限制和容忍度的定义，并减少从多个相关和不相关的研究领域的文献中提取生物体数据的需求。因此，我们开发了 ThermoBase，这是一个基于网络的免费数据库，目前包含了 1238 种嗜热或超嗜热生物体的综合描述。ThermoBase 报告了分类学、代谢、环境、实验和生理信息，以及文献资源。这包括化学渗透偶联离子、最佳 pH 值和范围、最佳温度和范围、最佳压力和最佳盐度等参数。数据库接口允许进行搜索功能和参数排序。因此，ThermoBase 的目标是在一个可访问和集中的存储库中，为科学界内的嗜热菌和超嗜热菌的假说生成、文献研究和理解提供便利和加速。ThermoBase 可免费在线访问，网址为天体生物学可居住环境数据库（AHED；https://ahed.nasa.gov）、生命科学数据库中心（TogoDB；http://togodb.org/db/thermobase）和 S1 文件。
