Wang Yulu, Du Xin, Wang Xuan, Yan Tingxiu, Yuan Mengqi, Yang Yuemeng, Jurado-Sánchez Beatriz, Escarpa Alberto, Xu Li-Ping
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Sciences, University of Alcalá, Madrid 28805, Spain.
ACS Appl Mater Interfaces. 2022 May 25;14(20):23129-23138. doi: 10.1021/acsami.1c24821. Epub 2022 May 10.
The slippery liquid-infused surfaces show a great antibacterial property. However, most liquid-infused surfaces cannot detect whether or not the unknown aqueous samples contain microorganisms. Therefore, it is highly necessary but a challenge to integrate bacterial sensing capability into antibacterial surface. In this work, we prepared a slippery patterned liquid-infused nanocoating on the glass substrate for integrating bacterial sensing capability into the bacterial repellence surface. Dendritic mesoporous silica nanoparticles (DMSNs) with a suitable particle size of ca. 128 nm were employed as a building block to fabricate the multifunctional nanocoating with a superhydrophilic microwell and hydrophobic periphery by a dip-coating strategy, hydrophobic treatment, photomask-mediated plasma etching, and liquid infusion. Dendritic porous silica nanoparticles (DPSNs) with a larger particle size of ca. 260 nm were uniformly loaded with Au nanoparticles (NPs), providing large surface area for the modification of Raman reporter (4-mercaptobenzoic acid (4-MBA)) and aptamer. Thus, as a Raman tag, the formed DPSNs-Au-MBA-aptamer could achieve sensitive surface-enhanced Raman spectroscopy (SERS) detection of target bacteria. Combined with the Raman tag, the patterned liquid-infused nanocoating not only completely repelled bacteria on the hydrophobic area but also enabled sensitive SERS detection of in a very low sample volume (1 μL) with a low detection limit of 2.6 colony formation units (CFU)/mL on the antibody-modified superhydrophilic microwell. This research provided a novel and reliable strategy to construct a multifunctional nanocoating with microbial repellence and sensing capabilities.
具有滑润液体的表面展现出很强的抗菌性能。然而，大多数具有液体的表面无法检测未知水性样品中是否含有微生物。因此，将细菌传感能力集成到抗菌表面是非常必要的，但也是一项挑战。在这项工作中，我们在玻璃基板上制备了一种具有图案的滑润液体注入纳米涂层，以便将细菌传感能力集成到细菌排斥表面。使用粒径约为128 nm的树枝状介孔二氧化硅纳米颗粒（DMSN）作为构建单元，通过浸涂策略、疏水处理、光掩模介导的等离子体蚀刻和液体注入，制备了具有超亲水微孔和疏水周边的多功能纳米涂层。粒径约为260 nm的较大尺寸树枝状多孔二氧化硅纳米颗粒（DPSN）均匀负载有金纳米颗粒（NP），为拉曼报告分子（4-巯基苯甲酸（4-MBA））和适配体的修饰提供了大表面积。因此，作为拉曼标签，形成的DPSN-Au-MBA-适配体能够实现对目标细菌的灵敏表面增强拉曼光谱（SERS）检测。结合拉曼标签，图案化的液体注入纳米涂层不仅在疏水区域完全排斥细菌，而且能够在抗体修饰的超亲水微孔上以非常低的样品体积（1 μL）实现灵敏的SERS检测，检测限低至2.6菌落形成单位（CFU）/mL。这项研究提供了一种新颖且可靠的策略来构建具有微生物排斥和传感能力的多功能纳米涂层。
