Zhang Xi, Xu Na-Yan, Ruan Qiong, Lu Dan-Qing, Yang Yun-Hui, Hu Rong
College of Chemistry and Chemical Engineering, Yunnan Normal University Kunming 650092 Yunnan P. R. China
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Molecular Engineering for Theronastics, Hunan University Changsha 410082 China.
RSC Adv. 2018 Feb 2;8(11):5714-5720. doi: 10.1039/c7ra12222e.
The rapid detection of pollutants with high sensitivity and selectivity is of considerable significance for security screening, environmental safety, and human health. In this study, we prepared persistent luminescence nanoparticles (PLNPs) and constructed a label-free sensor for sensitive and selective detection of pollutants in real samples and test papers. Following excitation, PLNPs could store absorbed light energy and release it in the form of luminescence. Compared with a fluorescence-based technique, a PLNPs-based measurement could effectively avoid background interference. Under optimal conditions, the limit of detection for TNP was found to be 10 nM, while for an antibiotic it was 5 nM. The nanoprobe was successfully applied for the detection of pollutants in real samples including milk and Dianchi Lake water samples. Due to the long-lasting afterglow nature of PLNPs, the signal-to-noise ratio could be greatly increased in complex real samples. By hand-writing with TNP solution as ink on filter paper, the photoluminescence (PL) of the part stained with TNP was immediately quenched. Moreover, after direct exposure under a UV lamp for 10 min and without further excitation, the luminescence of the test paper was investigated to avoid interferents. This PLNP material could be potentially employed as a multi-responsive luminescent sensor. In addition, these easy-to-use visual techniques could provide a powerful tool for a convenient POC assay of organic pollutants.
对污染物进行高灵敏度和高选择性的快速检测对于安全筛查、环境安全和人类健康具有相当重要的意义。在本研究中，我们制备了长余辉纳米颗粒（PLNPs），并构建了一种无标记传感器，用于灵敏且选择性地检测实际样品和试纸中的污染物。在激发后，PLNPs能够存储吸收的光能并以发光的形式释放出来。与基于荧光的技术相比，基于PLNPs的测量能够有效避免背景干扰。在最佳条件下，发现对三硝基苯酚（TNP）的检测限为10 nM，而对抗生素的检测限为5 nM。该纳米探针已成功应用于检测包括牛奶和滇池水样在内的实际样品中的污染物。由于PLNPs具有持久的余辉特性，在复杂的实际样品中，信噪比可大大提高。用TNP溶液作为墨水在滤纸上手写后，被TNP染色部分的光致发光（PL）立即淬灭。此外，在紫外灯下直接照射10分钟且无需进一步激发后，对试纸的发光情况进行了研究以避免干扰物。这种PLNP材料有可能用作多响应发光传感器。此外，这些易于使用的可视化技术可为方便地进行有机污染物的即时检测（POC）提供有力工具。
