Functionality Evaluation Research Group, Korea Food Research Institute, Seongnam 463-746, Korea.
Molecules. 2013 Aug 28;18(9):10404-12. doi: 10.3390/molecules180910404.
Puerariae radix, the dried root of Pueraria lobata Ohwi, is one of earliest and most important edible crude herbs used for various medical purposes in Oriental medicine. The aim of the present study was to determine the anti-inflammatory effects of Total Isoflavones from P. lobata (TIPL), which contains the unique isoflavone puerarin, in ischemia in vivo models. Oral administration of TIPL (100 mg/kg) reduced the brain infarct volume and attenuated ischemia-induced cyclooxygenase-2 (COX-2) up-regulation at 2 days after middle cerebral artery occlusion (MCAo) in rats. Moreover, TIPL reduced activation of glial fibrillary acid protein (GFAP) and CD11b antibody (OX-42) at 7 days after MCAo in hippocampal CA1 region. These results show that TIPL can protect the brain from ischemic damage after MCAo. Regarding the immunohistochemical study, the effects of TIPL may be attributable to its anti-inflammatory properties by the inhibition of COX-2 expression, astrocyte expression, and microglia.
葛根，为豆科植物野葛的干燥根，是东方医学中最早也是最重要的用于各种医疗用途的食用生草药之一。本研究旨在确定含有独特异黄酮葛根素的野葛总异黄酮（TIPL）在体内缺血模型中的抗炎作用。TIPL（100mg/kg）口服给药可减少大脑中动脉闭塞（MCAo）后 2 天大鼠的脑梗死体积，并减轻缺血诱导的环氧化酶-2（COX-2）上调。此外，TIPL 可减少 MCAo 后 7 天海马 CA1 区胶质纤维酸性蛋白（GFAP）和 CD11b 抗体（OX-42）的激活。这些结果表明 TIPL 可以保护大脑免受 MCAo 后的缺血损伤。关于免疫组织化学研究，TIPL 的作用可能与其通过抑制 COX-2 表达、星形胶质细胞表达和小胶质细胞来抑制炎症的特性有关。
