Diao H, Yan H L, Xiao Y, Yu B, Zheng P, He J, Yu J, Mao X B, Chen D W
Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Xinkang Road 46# Ya'an Sichuan Province 625014 People's Republic of China
Institute of Animal Nutrition, Sichuan Academy of Animal Science No. 7 Niusha Road Chengdu Sichuan 610066 People's Republic of China.
RSC Adv. 2018 Feb 27;8(16):8709-8720. doi: 10.1039/c7ra11234c. eCollection 2018 Feb 23.
The present study was conducted to investigate the effects of early fecal microbiota transplantation on gut development in sucking piglets. A total of 24 3 day-old DLY sucking piglets (2.11 ± 0.15) kg were randomly divided into four groups (TMP, YMP, RMP and control group (CON)), which were transplanted with intact fecal microbiota of Tibetan pig (TP), Yorkshire pig (YP), Rongchang pig (RP), and without transplantation, respectively. The whole trial lasted for 56 d. The results are as follows: when compared with the YMP and RMP treatments, TMP and CON had a lower diarrhea index ( < 0.05), TMP and CON had higher GLP-2 and ANG4 mRNA abundances in the ileum ( < 0.05), and the TMP had a higher jejunal villus height: crypt depth and a higher colonic GLP-2 mRNA abundance ( < 0.05). Moreover, when compared with the YMP and RMP treatments, TMP had an enhanced DMT1 mRNA abundance in the duodenum ( < 0.05), TMP and CON had a greater lactase activity and a higher DMT1 mRNA abundance in the jejunum ( < 0.05), and CON had a higher γ-GT activity in the jejunum ( < 0.05). The jejunal Ca, Mg-ATPase activity in TMP was higher than that in CON, and the jejunal Na, K-ATPase activity in TMP was higher than that in the other three treatments ( < 0.05). Besides, when compared with the YMP and RMP treatments, TMP had a lower MDA content and a higher MUC1 mRNA abundance in the jejunum ( < 0.05); CON had a higher SOD activity in the jejunum ( < 0.05), whereas TMP and CON had a higher butyric acid concentration in the colon and a lower LPS content in the serum ( < 0.05). Finally, when compared with the TMP treatment, the other three treatments had an enhanced IL-10 mRNA abundance in the colon ( < 0.05), YMP and CON had higher counts of in the colonic digesta ( < 0.05), and the CON had lower counts of spp in the cecal and colonic digesta ( < 0.05). These data indicated that early transplantation of the fecal microbiota from the Yorkshire pigs and Rongchang pigs to DLY suckling piglets would destroy the gut microbiota balance and thus damage intestinal health.
本研究旨在探讨早期粪便微生物群移植对哺乳仔猪肠道发育的影响。选取24头3日龄、体重（2.11±0.15）kg的DLY哺乳仔猪，随机分为四组（藏猪组（TMP）、大白猪组（YMP）、荣昌猪组（RMP）和对照组（CON）），分别移植藏猪、大白猪、荣昌猪的完整粪便微生物群，对照组不进行移植。整个试验持续56天。结果如下：与YMP和RMP组相比，TMP组和CON组腹泻指数较低（P<0.05），TMP组和CON组回肠中GLP-2和ANG4 mRNA丰度较高（P<0.05），TMP组空肠绒毛高度与隐窝深度比值及结肠GLP-2 mRNA丰度较高（P<0.05）。此外，与YMP和RMP组相比，TMP组十二指肠中DMT1 mRNA丰度升高（P<0.05），TMP组和CON组空肠乳糖酶活性较高且DMT1 mRNA丰度较高（P<0.05），CON组空肠γ-GT活性较高（P<0.05）。TMP组空肠Ca、Mg-ATP酶活性高于CON组，TMP组空肠Na、K-ATP酶活性高于其他三组（P<0.05）。此外，与YMP和RMP组相比，TMP组空肠MDA含量较低且MUC1 mRNA丰度较高（P<0.05）；CON组空肠SOD活性较高（P<0.05），而TMP组和CON组结肠丁酸浓度较高且血清LPS含量较低（P<0.05）。最后，与TMP组相比，其他三组结肠中IL-10 mRNA丰度升高（P<0.05），YMP组和CON组结肠食糜中大肠杆菌数量较多（P<0.05），CON组盲肠和结肠食糜中双歧杆菌数量较少（P<0.05）。这些数据表明，早期将大白猪和荣昌猪的粪便微生物群移植到DLY哺乳仔猪体内会破坏肠道微生物群平衡，进而损害肠道健康。
