Xu Junhuai, Yan Rui, Wang Haibo, Du Zongliang, Gu Jun, Cheng Xu, Xiong Junjie
Textile Institute, College of Light Industry, Textile and Food Engineering, Sichuan University Chengdu 610065 China
Department of Cardiovascular Surgery, West China Hospital, Sichuan University Chengdu Sichuan 610041 China.
RSC Adv. 2018 Feb 12;8(13):6798-6804. doi: 10.1039/c7ra13238g. eCollection 2018 Feb 9.
In this work, a novel and stable zwitterionic polymer (TPE-CB PUs) was prepared to realize a cellular imaging system. TPE was conjugated into the backbone of zwitterionic polyurethane, which could be well dispersed in aqueous solution and emitted strong blue fluorescence because the TPE segment was aggregated in the core of TPE-CB PUs micelles. More importantly, the TPE-CB PUs micelles showed significant stability in a large pH window and with different storage times. In addition, the micelles exhibited low cytotoxicity in HeLa cells and mainly distributed in the cytoplasm after being incubated with cells. The outstanding properties of TPE-CB PUs combining the merits of AIE and a zwitterionic segment highlight its potential for use as a cell imaging material with remarkable capability.
在这项工作中，制备了一种新型且稳定的两性离子聚合物（TPE-CB聚氨酯）以实现细胞成像系统。四苯乙烯（TPE）共轭到两性离子聚氨酯的主链中，由于TPE链段聚集在TPE-CB聚氨酯胶束的核心，该聚合物能很好地分散在水溶液中并发出强烈的蓝色荧光。更重要的是，TPE-CB聚氨酯胶束在较大的pH窗口和不同的储存时间下均表现出显著的稳定性。此外，该胶束在HeLa细胞中表现出低细胞毒性，与细胞孵育后主要分布在细胞质中。TPE-CB聚氨酯结合了聚集诱导发光（AIE）和两性离子链段的优点，其优异性能突出了其作为具有卓越能力的细胞成像材料的潜力。
