Chou Ying-Nien, Sun Fang, Hung Hsiang-Chieh, Jain Priyesh, Sinclair Andrew, Zhang Peng, Bai Tao, Chang Yung, Wen Ten-Chin, Yu Qiuming, Jiang Shaoyi
Department of Chemical Engineering, University of Washington, Seattle, WA 98195-1750, USA; Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Department of Chemical Engineering, University of Washington, Seattle, WA 98195-1750, USA.
Acta Biomater. 2016 Aug;40:31-37. doi: 10.1016/j.actbio.2016.04.023. Epub 2016 Apr 16.
For surface-based diagnostic devices to achieve reliable biomarker detection in complex media such as blood, preventing nonspecific protein adsorption and incorporating high loading of biorecognition elements are paramount. In this work, a novel method to produce nonfouling zwitterionic hydrogel coatings was developed to achieve these goals. Poly(carboxybetaine acrylamide) (pCBAA) hydrogel thin films (CBHTFs) prepared with a carboxybetaine diacrylamide crosslinker (CBAAX) were coated on gold and silicon dioxide surfaces via a simple spin coating process. The thickness of CBHTFs could be precisely controlled between 15 and 150nm by varying the crosslinker concentration, and the films demonstrated excellent long-term stability. Protein adsorption from undiluted human blood serum onto the CBHTFs was measured with surface plasmon resonance (SPR). Hydrogel thin films greater than 20nm exhibited ultra-low fouling (<5ng/cm(2)). In addition, the CBHTFs were capable of high antibody functionalization for specific biomarker detection without compromising their nonfouling performance. This strategy provides a facile method to modify SPR biosensor chips with an advanced nonfouling material, and can be potentially expanded to a variety of implantable medical devices and diagnostic biosensors.
In this work, we developed an approach to realize ultra-low fouling and high ligand loading with a highly-crosslinked, purely zwitterionic, carboxybetaine thin film hydrogel (CBHTF) coating platform. The CBHTF on a hydrophilic surface demonstrated long-term stability. By varying the crosslinker content in the spin-coated hydrogel solution, the thickness of CBHTFs could be precisely controlled. Optimized CBHTFs exhibited ultra-low nonspecific protein adsorption below 5ng/cm(2) measured by a surface plasmon resonance (SPR) sensor, and their 3D architecture allowed antibody loading to reach 693ng/cm(2). This strategy provides a facile method to modify SPR biosensor chips with an advanced nonfouling material, and can be potentially expanded to a variety of implantable medical devices and diagnostic biosensors.
对于基于表面的诊断设备而言，要在血液等复杂介质中实现可靠的生物标志物检测，防止非特异性蛋白质吸附并纳入高负载的生物识别元件至关重要。在这项工作中，开发了一种制备抗污两性离子水凝胶涂层的新方法来实现这些目标。用羧基甜菜碱二丙烯酰胺交联剂（CBAAX）制备的聚（羧基甜菜碱丙烯酰胺）（pCBAA）水凝胶薄膜（CBHTFs）通过简单的旋涂工艺涂覆在金和二氧化硅表面。通过改变交联剂浓度，CBHTFs的厚度可以精确控制在15至150纳米之间，并且这些薄膜表现出优异的长期稳定性。用表面等离子体共振（SPR）测量了未稀释的人血清中蛋白质在CBHTFs上的吸附情况。厚度大于20纳米的水凝胶薄膜表现出超低污垢（<5纳克/平方厘米）。此外，CBHTFs能够进行高抗体功能化以用于特异性生物标志物检测，而不会损害其抗污性能。该策略提供了一种用先进的抗污材料修饰SPR生物传感器芯片的简便方法，并且有可能扩展到各种可植入医疗设备和诊断生物传感器。
在这项工作中，我们开发了一种方法，通过高度交联、纯两性离子的羧基甜菜碱薄膜水凝胶（CBHTF）涂层平台实现超低污垢和高配体负载。亲水性表面上的CBHTF表现出长期稳定性。通过改变旋涂水凝胶溶液中的交联剂含量，可以精确控制CBHTFs的厚度。经表面等离子体共振（SPR）传感器测量，优化后的CBHTFs表现出低于5纳克/平方厘米的超低非特异性蛋白质吸附，并且其三维结构使抗体负载量达到693纳克/平方厘米。该策略提供了一种用先进的抗污材料修饰SPR生物传感器芯片的简便方法，并且有可能扩展到各种可植入医疗设备和诊断生物传感器。
