Du Hongying, Fu Jialing, Wang Siqi, Liu Huili, Zeng Yongchao, Yang Jiaren, Xiong Shanbai
College of Food Science and Technology, Huazhong Agricultural University Wuhan Hubei 430070 P. R. China
National R & D Branch Center for Conventional Freshwater Fish Processing Wuhan Hubei 430070 P. R. China.
RSC Adv. 2018 May 29;8(35):19470-19478. doi: 10.1039/c8ra02311e. eCollection 2018 May 25.
There are a number of bioactive compounds in freshwater fish brains, and their functional roles have not been clearly elucidated. NMR-based metabolic profiling could enable rapid characterization of the nutritional composition a fish's brain. Here, two kinds of freshwater fish brains were investigated, crucian carp and yellow catfish. A H-NMR based metabolomic approach was used to illustrate the nutritional components of these two kinds of brain. At first, the microwave method was utilized to cease the activity of the enzymes in the brain, and the chemicals were extracted for NMR analysis. These two kinds of brain had significant differences in metabolic patterns, and the chemical compositions of the yellow catfish brain were similar to those of rodent and human brains. Furthermore, most of the different metabolites were significantly higher in the yellow catfish, except for acetamide. This study could provide comprehensive information regarding the utilization of fish heads during processing of fish and dietary nutrition guidance.
淡水鱼脑中存在多种生物活性化合物，但其功能作用尚未得到明确阐释。基于核磁共振的代谢谱分析能够快速表征鱼脑的营养成分。在此，对两种淡水鱼脑进行了研究，即鲫鱼和黄颡鱼。采用基于氢核磁共振的代谢组学方法来阐明这两种鱼脑的营养成分。首先，利用微波法终止脑内酶的活性，然后提取化学物质用于核磁共振分析。这两种鱼脑的代谢模式存在显著差异，黄颡鱼脑的化学成分与啮齿动物和人类的脑相似。此外，除了乙酰胺外，大多数不同的代谢物在黄颡鱼脑中的含量显著更高。本研究可为鱼类加工过程中鱼头的利用及膳食营养指导提供全面信息。
