UCD Institute of Food and Health, Belfield, UCD, Dublin 4, Ireland.
Prog Nucl Magn Reson Spectrosc. 2014 Nov;83:42-9. doi: 10.1016/j.pnmrs.2014.09.001. Epub 2014 Nov 5.
Metabolomics is the study of metabolites present in biological samples such as biofluids, tissue/cellular extracts and culture media. Measurement of these metabolites is achieved through use of analytical techniques such as NMR and mass spectrometry coupled to liquid chromatography. Combining metabolomic data with multivariate data analysis tools allows the elucidation of alterations in metabolic pathways under different physiological conditions. Applications of NMR-based metabolomics have grown in recent years and it is now widely used across a number of disciplines. The present review gives an overview of the developments in the key steps involved in an NMR-based metabolomics study. Furthermore, there will be a particular emphasis on the use of NMR-based metabolomics in nutrition research.
代谢组学是研究生物样本中存在的代谢物的科学，例如生物体液、组织/细胞提取物和培养基。这些代谢物的测量是通过使用分析技术（如 NMR 和与液相色谱耦联的质谱）来实现的。将代谢组学数据与多元数据分析工具相结合，可以阐明不同生理条件下代谢途径的变化。基于 NMR 的代谢组学的应用近年来有所增加，现在已广泛应用于多个学科。本综述概述了基于 NMR 的代谢组学研究中涉及的关键步骤的发展。此外，还将特别强调基于 NMR 的代谢组学在营养研究中的应用。
