Jeon Jinseong, Park Seung Cheol, Her Jin, Lee Jae Won, Han Jin-Kwan, Kim Yoon-Keun, Kim Kwang Pyo, Ban Changill
Department of Life Sciences, Pohang University of Science and Technology (POSTECH) 77, Cheongam-Ro, Nam-Gu Pohang Gyeongbuk 37673 Republic of Korea.
Department of Applied Chemistry, College of Applied Science, Kyung Hee University Yongin 17104 Republic of Korea.
RSC Adv. 2018 Apr 23;8(27):15241-15247. doi: 10.1039/c7ra13769a. eCollection 2018 Apr 18.
is a lipophilic commensal bacterium mainly found on the skin and in the gastrointestinal tract. Pathophysiological effects of have recently been reported not only in acne progression but in various diseases. As an emerging mode of bacterial communication, extracellular vesicles (EVs) have been demonstrated to conduct critical pathophysiological functions. To provide information on lipid composition for the first time, we conducted a comparative lipidomic analysis of and EVs and identified 214 lipids with high confidence using triplicated liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analyses. EVs contained substantially more PCs, DGs, PAs, PEs, LPAs, LPCs, and MGs than , and contained fewer PSs, SO1Ps, SA1Ps, LPGs, LPIs, and LPSs. Distinctively, EVs possessed a markedly reduced amount of TG. These findings will provide useful clues for understanding the biological and pathophysiological mechanisms of and for clinical applications such as vaccine development, diagnostics and therapeutics.
是一种亲脂性共生细菌，主要存在于皮肤和胃肠道中。最近报道了其病理生理效应，不仅在痤疮进展中，而且在各种疾病中。作为一种新兴的细菌通讯模式，细胞外囊泡（EVs）已被证明具有关键的病理生理功能。为首次提供有关其脂质组成的信息，我们对其和EVs进行了比较脂质组学分析，并使用三重液相色谱-串联质谱（LC-MS/MS）分析高可信度地鉴定了214种脂质。其EVs比其含有更多的PC、DG、PA、PE、LPA、LPC和MG，而含有较少的PS、SO1P、SA1P、LPG、LPI和LPS。特别地，其EVs的TG含量明显降低。这些发现将为理解其生物学和病理生理机制以及疫苗开发、诊断和治疗等临床应用提供有用线索。
