一种用于淀粉样纤维检测的灵敏探针，具有强荧光和早期响应。

A sensitive probe for amyloid fibril detection with strong fluorescence and early response.

作者信息

Zheng Xiaolin, Xu Zhenzhen, Li Haiyang, Fu Hongbing

机构信息

Beijing Key Laboratory for Optical Materials and Photonic Devices, Department of Chemistry, Capital Normal University Beijing 100048 People's Republic of China

College of Life Sciences, Capital Normal University Beijing 100048 People's Republic of China.

出版信息

RSC Adv. 2018 Apr 27;8(29):15870-15875. doi: 10.1039/c8ra00751a.

DOI:10.1039/c8ra00751a

PMID:35542196

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9080103/

Abstract

We synthesized a new probe, 4-[2-(2-naphthyl)-()-ethenyl]-benzyl(triphenyl)phosphonium bromide (NEB), to detect the formation of amyloid fibrils of bovine insulin. The fluorescence intensity of NEB in the presence of insulin fibrils was 30 times higher than that before fibrillation, with the fluorescence quantum yield increased from 2.5% to 78%. In comparison with the commercially available probe, thioflavin T (ThT), NEB exhibits a 10 times stronger fluorescence and a shorter identification lag phase for detecting insulin fibrillation, indicating a higher sensitivity in detection of insulin oligomers and fibrils.

摘要

我们合成了一种新的探针，4-[2-(2-萘基)-（E）-乙烯基]-苄基（三苯基）溴化膦（NEB），用于检测牛胰岛素淀粉样纤维的形成。在胰岛素纤维存在的情况下，NEB的荧光强度比纤维化前高30倍，荧光量子产率从2.5%提高到78%。与市售探针硫黄素T（ThT）相比，NEB在检测胰岛素纤维化时表现出强10倍的荧光和更短的识别延迟期，表明其在检测胰岛素寡聚体和纤维方面具有更高的灵敏度。