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利用化学探针实现芬顿反应中羟基自由基的可靠定量分析。

Towards reliable quantification of hydroxyl radicals in the Fenton reaction using chemical probes.

作者信息

Rutely C Burgos Castillo, Jean-M Fontmorin, Walter Z Tang, Xochitl Dominguez-Benetton, Mika Sillanpää

机构信息

Laboratory of Green Chemistry, School of Engineering Science, Lappeenranta University of Technology Sammonkatu 12, FI-50130 Mikkeli Finland

Separation and Conversion Technologies, Flemish Institute for Technological Research (VITO) Boeretang 200, 2400, Mol Belgium.

出版信息

RSC Adv. 2018 Jan 31;8(10):5321-5330. doi: 10.1039/c7ra13209c. eCollection 2018 Jan 29.

DOI:10.1039/c7ra13209c
PMID:35542446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9078104/
Abstract

Quantification of hydroxyl radical concentration using two chemical probes was assessed through the Fenton reaction. The probes were 1,2-benzopyrone (coumarin) for fluorescence and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) for electron spin resonance (ESR). The corresponding hydroxylated species, namely 7-hydroxycoumarin (7HC) and 2-hydroxy-5,5-dimethyl-1-pyrroline-N-oxide (DMPO-OH adduct), were monitored by fluorescence and ESR-spin trapping techniques, respectively. The experiments were designed according to the theoretical conditions determined for stable fluorescence and EPR signals. The results demonstrate that: the optimal [chemical probe] : [HO] ratio predicted by a simplified quasi-steady-state model was in good agreement with the optimal [chemical probe] : [HO] ratio observed experimentally for [HO] : [Fe] = 10, and the proper adjustment of the [chemical probe] : [HO] ratio at a given concentration of the Fenton's reagent improves the detected amount of hydroxyl radicals. Finally, using DMPO required a higher concentration compared to coumarin to yield the same amount of ˙OH detected but resulted in a more reliable probe for detecting ˙OH under the consideration of this study.

摘要

通过芬顿反应评估了使用两种化学探针定量羟基自由基浓度的方法。用于荧光检测的探针是1,2 - 苯并吡喃（香豆素），用于电子自旋共振（ESR）检测的探针是5,5 - 二甲基 - 1 - 吡咯啉 - N - 氧化物（DMPO）。分别通过荧光和ESR自旋捕获技术监测相应的羟基化产物，即7 - 羟基香豆素（7HC）和2 - 羟基 - 5,5 - 二甲基 - 1 - 吡咯啉 - N - 氧化物（DMPO - OH加合物）。实验是根据确定稳定荧光和EPR信号的理论条件设计的。结果表明：简化的准稳态模型预测的最佳[化学探针]∶[HO]比率与[HO]∶[Fe] = 10时实验观察到的最佳[化学探针]∶[HO]比率高度吻合，并且在给定浓度的芬顿试剂下适当调整[化学探针]∶[HO]比率可提高检测到的羟基自由基量。最后，与香豆素相比，使用DMPO产生相同量的检测到的˙OH需要更高的浓度，但在本研究中考虑，DMPO是检测˙OH更可靠的探针。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/4406973705a7/c7ra13209c-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/0d14977d88f4/c7ra13209c-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/3a6240f0714f/c7ra13209c-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/319cb61a1095/c7ra13209c-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/6f4d1291e930/c7ra13209c-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/4406973705a7/c7ra13209c-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/0d14977d88f4/c7ra13209c-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/3a6240f0714f/c7ra13209c-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/319cb61a1095/c7ra13209c-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/6f4d1291e930/c7ra13209c-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/123c/9078104/4406973705a7/c7ra13209c-f5.jpg

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