Suomalainen-Nevanlinna H A, Schröder J, Andersson L C
Scand J Immunol. 1987 Apr;25(4):421-3. doi: 10.1111/j.1365-3083.1987.tb02209.x.
Leu-M2 (Mac-120) antigen is a cell-surface marker present on a subpopulation of human blood monocytes and platelets. Its expression on monocytes and macrophages is necessary for their ability to present antigen to T lymphocytes. Only Leu-M2+ cells display the human Ia-like determinant, HLA-DS, which seems important in antigen presentation and stimulation in the autologous MLR. Expression of the Leu-M2/Mac-120 antigen was studied by indirect immunofluorescence analysis and flow cytofluorometry in mouse-human somatic cell hybrids segregating human chromosomes. In the mouse-human hybrid clones, expression of the Leu-M2/Mac-120 antigen was dependent on the presence of human chromosome 11. This was verified by fluorescence-activated cell sorting of heterogeneous hybrid cell populations into antigen-positive and-negative fractions. The Leu-M2 antigen co-segregated with chromosome 11. The gene controlling the expression of the Leu-M2/Mac-120 antigen thus is assigned to human chromosome 11.
亮氨酸M2（Mac-120）抗原是一种存在于人类血液单核细胞和血小板亚群上的细胞表面标志物。其在单核细胞和巨噬细胞上的表达对于它们向T淋巴细胞呈递抗原的能力是必需的。只有Leu-M2+细胞显示出人类Ia样决定簇HLA-DS，这在自体混合淋巴细胞反应中的抗原呈递和刺激中似乎很重要。通过间接免疫荧光分析和流式细胞荧光测定法，对分离人类染色体的小鼠-人类体细胞杂种中的Leu-M2/Mac-120抗原表达进行了研究。在小鼠-人类杂种克隆中，Leu-M2/Mac-120抗原的表达取决于人类11号染色体的存在。通过将异质杂种细胞群体荧光激活细胞分选成抗原阳性和阴性部分，证实了这一点。Leu-M2抗原与11号染色体共分离。因此，控制Leu-M2/Mac-120抗原表达的基因被定位于人类11号染色体。
