Schröder J, Nikinmaa B, Kavathas P, Herzenberg L A
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3421-4. doi: 10.1073/pnas.80.11.3421.
Leu 7 (HNK-1) is a membrane antigen expressed on human natural killer cells and some other lymphoid cells. Starting with two clones of mouse-human hybrid lymphoid cells that had 1.6% and 35% Leu 7-positive cells, respectively, we viably sorted Leu 7-positive and -negative cells using a fluorescence-activated cell sorter (FACS). Short-term progeny of the sorted cells were then karyotyped. Chromosome 11 was the only human chromosome that was absent from the Leu 7-negative population and present in nearly all of the progeny of the Leu 7-positive selected cells. Thus, we assigned the Leu 7 gene to chromosome 11.
Leu 7（HNK-1）是一种在人类自然杀伤细胞和其他一些淋巴细胞上表达的膜抗原。从分别含有1.6%和35% Leu 7阳性细胞的两株小鼠-人杂交淋巴细胞克隆开始，我们使用荧光激活细胞分选仪（FACS）对Leu 7阳性和阴性细胞进行了活细胞分选。然后对分选细胞的短期子代进行了核型分析。11号染色体是Leu 7阴性群体中唯一缺失且几乎存在于所有Leu 7阳性分选细胞子代中的人类染色体。因此，我们将Leu 7基因定位于11号染色体。
