Peters P G, Kamarck M E, Hemler M E, Strominger J L, Ruddle F H
Somatic Cell Genet. 1982 Nov;8(6):825-34. doi: 10.1007/BF01543022.
We have mapped the gene which codes the species-specific determinant defined by monoclonal antibody 4F2 to human chromosome 11. All human chromosomes, except Y, were included in a group of four human-mouse hybrid lines. Hybrids heterogeneous for 4F2 antigen expression were sorted using the fluorescence-activated cell sorter (FACS) to yield populations homogeneous with respect to the presence or absence of this determinant. Isozyme analysis indicated corresponding genetic selection for or against human chromosome 11. This map assignment was confirmed using a hybrid line which contained only human chromosome 11. Immunoprecipitation of the 4F2 determinant from the 11 only hybrid resulted in a heavy subunit of molecular weight (Mr) = 100,000 and a light subunit of Mr = 41,000. This contrasts with results obtained from nonhybrid human cells of different lineages. These results demonstrate the importance of FACS techniques in the rapid mapping of genes which code human cell surface antigens.
我们已将编码由单克隆抗体4F2定义的物种特异性决定簇的基因定位到人类11号染色体上。除Y染色体外的所有人类染色体都包含在一组四个人-鼠杂交细胞系中。使用荧光激活细胞分选仪（FACS）对4F2抗原表达异质的杂交细胞进行分选，以产生关于该决定簇存在与否均一的群体。同工酶分析表明对人类11号染色体进行了相应的正向或反向遗传选择。使用仅包含人类11号染色体的杂交细胞系证实了该图谱定位。从仅含11号染色体的杂交细胞中免疫沉淀4F2决定簇，得到分子量（Mr）为100,000的重链亚基和Mr为41,000的轻链亚基。这与从不同谱系的非杂交人类细胞获得的结果形成对比。这些结果证明了FACS技术在快速定位编码人类细胞表面抗原的基因方面的重要性。
