使用 Nanopore Flongle 对马来西亚本地品种猫肠道微生物组的 ITS1 扩增子进行测序。
ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle.
机构信息
Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia.
Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Pahang, Malaysia.
出版信息
Arch Microbiol. 2022 May 11;204(6):314. doi: 10.1007/s00203-022-02929-3.
The gut mycobiome exhibits major influence on the gastrointestinal health and disease but received less attention due to low abundance. This study characterizes the fungal community and compares the microbial diversity between indoor and outdoor cats. Genomic DNA was extracted and sequenced by targeting the Internal Transcribed Spacer 1 (ITS1) region using Flongle flow cell on MinION™ sequencing platform. Results show the phylum Ascomycota and genus Peniophorella were numerous in indoor cats, whereas the Basidiomycota and Pichia were abundant in outdoor cats. Peniophorella formed the core mycobiome in both feline populations. Furthermore, alpha (p value = 0.0207) and beta diversities (p value = 0.009) results showed significant differences between the two groups. Overall, indoor cats have greater amounts of Peniophorella, whereas outdoor cats have higher Trichosporon and unclassified Sordariaceae. The study also suggests that keeping a cat indoors or left as a stray will affect their respective gut mycobiome.
肠道真菌组对胃肠道健康和疾病有重要影响，但由于丰度低，受到的关注较少。本研究对室内和室外猫的真菌群落进行了特征描述，并比较了微生物多样性。使用 Flongle 流池，通过靶向内部转录间隔区 1（ITS1）区域，在 MinION 测序平台上对基因组 DNA 进行提取和测序。结果表明，在室内猫中，子囊菌门和 Peniophorella 属数量较多，而担子菌门和毕赤酵母属在室外猫中较为丰富。Peniophorella 在两种猫群中均形成核心真菌组。此外，α（p 值=0.0207）和β多样性（p 值=0.009）结果表明两组之间存在显著差异。总体而言，室内猫的 Peniophorella 含量较高，而室外猫的 Trichosporon 和未分类的 Sordariaceae 含量较高。该研究还表明，将猫养在室内或放任其流浪会影响它们各自的肠道真菌组。
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