探讨潜伏性弓形虫感染与住院患者 COVID-19 之间的关联：首个基于注册的研究。
Exploring the Association Between Latent Toxoplasma gondii Infection and COVID-19 in Hospitalized Patients: First Registry-Based Study.
机构信息
Toxoplasmosis Research Center, Communicable Diseases Institute, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, PO Box 48471-91971, Sari, Iran.
Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Acta Parasitol. 2022 Sep;67(3):1172-1179. doi: 10.1007/s11686-022-00559-9. Epub 2022 May 11.
PURPOSE
This study aimed to determine the possible association between Toxoplasma gondii infection and COVID-19 outcomes among 133 patients with an RT-PCR-positive test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hospitalized at Imam Khomeini Hospital, Sari, Mazandaran Province, northern Iran, during August to November 2020.
METHODS
A questionnaire was used to collect baseline data from the patients who were registered to the Iranian National Registry Center for Toxoplasmosis (INRCT). Also, blood samples were taken from each patient for detecting anti-T. gondii antibodies and T. gondii DNA using enzyme-linked immunosorbent assay (ELISA) and conventional-PCR methods, respectively. Variables related to the COVID-19 severity and outcomes were indicated based on multiple multinomial logistic regression models.
RESULTS
Of 133 patients enrolled in the INRCT with COVID-19 through RT-PCR, 50 (37.59%), 52 (39.1%), and 31 (23%) suffered from mild, moderate, and severe COVID-19, respectively. 57.1% of the patients who died had severe COVID-19, while among those with other outcomes, only 18.60% had severe COVID-19 (P < 0.05). Anti-T. gondii IgG was detected in 109/133 (81.95%) patients, which was not statistically significant (P > 0.05). Among those with negative and positive anti-T. gondii IgG, 2 (8.30%) and 29 (26.60%) had severe COVID-19, respectively (P > 0.05). T. gondii DNA and anti-T. gondii IgM were not found in any of the patients. Moreover, all deaths occurred in those with moderate or severe COVID-19 and a positive anti-T. gondii IgG.
CONCLUSION
To our knowledge, this is the first registry-based study concerning T. gondii infection among patients with COVID-19. Our data show the high rate of latent T. gondii infection among COVID-19 with different severity. However, there is no significant relationship between latent T. gondii infection and COVID-19 severity and outcomes. Thus, conducting multicenter studies in different geographic regions of the world could offer a better understanding of this relationship.
目的
本研究旨在确定在伊朗北部马赞达兰省萨里市伊玛目霍梅尼医院住院的 133 名严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）RT-PCR 阳性患者中，弓形体感染与 COVID-19 结局之间的可能关联。这些患者于 2020 年 8 月至 11 月期间登记在伊朗国家弓形体病登记中心（INRCT）。此外，从每位患者采集血样，分别使用酶联免疫吸附试验（ELISA）和常规 PCR 方法检测抗弓形体抗体和弓形体 DNA。根据多项多项多项逻辑回归模型，指出与 COVID-19 严重程度和结局相关的变量。
方法
使用问卷从登记在 INRCT 的 COVID-19 患者中收集基线数据。此外，从每位患者采集血样，分别使用酶联免疫吸附试验（ELISA）和常规 PCR 方法检测抗弓形体抗体和弓形体 DNA。根据多项多项多项逻辑回归模型，指出与 COVID-19 严重程度和结局相关的变量。
结果
在通过 RT-PCR 纳入 INRCT 的 133 名 COVID-19 患者中，50 名（37.59%）、52 名（39.1%）和 31 名（23%）分别患有轻度、中度和重度 COVID-19。57.1%的死亡患者患有严重 COVID-19，而在其他结局的患者中，只有 18.60%患有严重 COVID-19（P<0.05）。在 133 名患者中，有 109 名（81.95%）检测到抗弓形体 IgG，这没有统计学意义（P>0.05）。在抗弓形体 IgG 阴性和阳性的患者中，分别有 2 名（8.30%）和 29 名（26.60%）患有严重 COVID-19（P>0.05）。在任何患者中均未发现弓形体 DNA 和抗弓形体 IgM。此外，所有死亡均发生在患有中度或重度 COVID-19 和阳性抗弓形体 IgG 的患者中。
结论
据我们所知，这是第一项关于 COVID-19 患者弓形体感染的基于登记的研究。我们的数据显示，不同严重程度的 COVID-19 患者中存在较高比例的潜伏性弓形体感染。然而，潜伏性弓形体感染与 COVID-19 严重程度和结局之间没有显著关系。因此，在世界不同地理区域进行多中心研究可以更好地了解这种关系。
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