Department of Biology, College of Science, University of Misan, Maysan, Iraq.
Arch Razi Inst. 2021 Dec 30;76(6):1815-1822. doi: 10.22092/ari.2021.356357.1824. eCollection 2021 Dec.
One of the main factors which played a key role in the prevention of liver disorders such as hepatic inflammation, fibrosis, and carcinogenesis would be the vitamin D axis. Therefore, the current research was designed to evaluate the role of Vitamin D (Vit D) as a protective agent against liver damage caused by Thioacetamide (TAA). In the current study, 18 male Wistar rats were randomly allocated into three equal groups (n=6): in group 1(G1) the animals were considered as the control group and did not receive any supplement in drinking water; in group 2 (G2) TAA was administrated to the drinking water at a dose of 300 mg/L; in group 3 (G3) TAA was administrated to the drinking water at a dose of 300 mg/L plus vitamin D at a dose of 0.5 mg/100g body (intraperitoneal) for 8 weeks. At the end of the experiment, the animals were sacrificed and the liver was dissected and removed for histopathology. Histopathological evaluations were used to evaluate the possible adverse effects of TAA on the liver. Several hepatic damages were observed in the G2 group such as lobular disorder, some degrees of degeneration in hepatocytes and enlargement of the hepatic capillaries, and focal necrotic areas. Hepatic fibrosis was observed around portal areas and central veins. Bridging fibrous septa were formed between portal veins. The recorded data in this study showed that Vit D has some beneficial effects in protecting the liver from fibrosis and toxic damages. The recorded data showed that liver damages in the G3 group were partially prevented or cured. In conclusion, it is evident that the Vit D played a pivotal role as an antioxidant and anti-fibrotic agent, therefore it would be the best supplement for liver protection against damages due to toxin entrance into the animal's body.
维生素 D 轴在预防肝脏疾病（如肝炎症、纤维化和癌变）方面起着关键作用。因此，本研究旨在评估维生素 D (Vit D) 作为一种保护剂，防止硫代乙酰胺 (TAA) 引起的肝损伤。在本研究中，将 18 只雄性 Wistar 大鼠随机分为三组（n=6）：第 1 组（G1）作为对照组，饮用水中不添加任何补充剂；第 2 组（G2）在饮用水中添加 300mg/L 的 TAA；第 3 组（G3）在饮用水中添加 300mg/L 的 TAA 和 0.5mg/100g 体重的 Vit D（腹腔内），持续 8 周。实验结束时，处死动物，解剖肝脏并取出用于组织病理学检查。组织病理学评估用于评估 TAA 对肝脏的可能不良影响。在 G2 组中观察到几种肝损伤，如小叶紊乱、肝细胞不同程度变性和肝毛细血管扩张、局灶性坏死区。门脉周围和中央静脉观察到肝纤维化。门脉之间形成桥接纤维性间隔。本研究记录的数据表明，Vit D 对保护肝脏免受纤维化和毒性损伤具有一些有益作用。记录的数据表明，G3 组的肝损伤部分得到预防或治愈。总之，Vit D 作为一种抗氧化剂和抗纤维化剂发挥了关键作用，因此是保护肝脏免受毒素进入动物体内引起损伤的最佳补充剂。
