Khan Imran, Sivasankaran Nivetha, Nagarjuna Ravikiran, Ganesan Ramakrishnan, Dutta Jayati Ray
Department of Biological Sciences, BITS Pilani, Hyderabad Campus Jawahar Nagar, Kapra Mandal Hyderabad - 500078 Telangana India
Department of Chemistry, BITS Pilani, Hyderabad Campus Jawahar Nagar, Kapra Mandal Hyderabad - 500078 Telangana India
RSC Adv. 2018 Sep 6;8(55):31358-31365. doi: 10.1039/c8ra05999c. eCollection 2018 Sep 5.
The microbial resistance to different drugs due to excessive usage of antibiotics in various domains has become a serious environmental threat in recent years. This gave the impetus to researchers to find alternatives that do not lead to multi-drug resistant microbes. In this backdrop, silver nanoparticles (Ag NPs) have become a popular choice due to their potential broad spectrum of antimicrobial attributes. Recent literature caution that about 400 metric tons of Ag NPs are synthesized annually all over the world that could cause environmental hazards when used at higher concentrations than the toxicity limit. However, most of the literature reports use higher concentrations of Ag NPs and exposure to such concentrations may lead to environmental and health hazards. In this study, a series of Ag NPs have been synthesized using a lipase derived from a probiotic source as the stabilizing agent. The Ag NPs synthesized through different combinations of lipase and AgNO are characterized using various techniques such as UV-visible spectroscopy, FT-IR, ED-XRF, DLS and HR-TEM. The lipase capped Ag NPs have been studied for their antimicrobial activity against representative microbes such as , and . Our initial results reveal that the lipase capped Ag NPs possess high potential towards broad spectrum antimicrobial applications at concentrations much lower than the toxicity limit of the standard model, zebra fish.
近年来，由于抗生素在各个领域的过度使用，微生物对不同药物产生的耐药性已成为一个严重的环境威胁。这促使研究人员寻找不会导致多重耐药微生物产生的替代物。在此背景下，银纳米颗粒（Ag NPs）因其潜在的广谱抗菌特性而成为一种热门选择。最近的文献警告称，全球每年合成约400公吨的银纳米颗粒，当使用浓度高于毒性极限时可能会造成环境危害。然而，大多数文献报道使用的是较高浓度的银纳米颗粒，暴露于这样的浓度可能会导致环境和健康危害。在本研究中，使用源自益生菌源的脂肪酶作为稳定剂合成了一系列银纳米颗粒。通过脂肪酶和硝酸银的不同组合合成的银纳米颗粒，使用紫外可见光谱、傅里叶变换红外光谱、能量色散X射线荧光光谱、动态光散射和高分辨率透射电子显微镜等各种技术进行表征。对用脂肪酶包覆的银纳米颗粒针对代表性微生物如 、 和 的抗菌活性进行了研究。我们的初步结果表明，用脂肪酶包覆的银纳米颗粒在浓度远低于标准模型斑马鱼的毒性极限时，具有用于广谱抗菌应用的巨大潜力。
