Mazinani Sina Atrin, Stuart Jeffrey A, Yan Hongbin
Department of Chemistry, Brock University 1812 Sir Isaac Brock Way St. Catharines Ontario Canada L2S 3A1
Centre for Biotechnology, Brock University 1812 Sir Isaac Brock Way St. Catharines Ontario Canada L2S 3A1.
RSC Adv. 2018 Sep 7;8(55):31465-31470. doi: 10.1039/c8ra05605f. eCollection 2018 Sep 5.
Exposure of MCF-7 breast and PC-3 prostate cancer cells to 10 W microwaves at 2.45 GHz increased their uptake of the cancer drug doxorubicin from media by almost 100%, concomitantly increasing cell death, while microwave exposure alone had no cellular toxicity. Addition of inhibitors of endocytosis during the treatment of MCF-7 cells with doxorubicin and microwaves showed no impact on the uptake of the anticancer drug. Furthermore, the uptake of oligonucleotides by MCF-7 cells is not affected by the treatment with microwaves. These observations suggest that endocytosis is not involved in the uptake of doxorubicin while cells are exposed to microwave irradiation. Thus, targeted low power microwave irradiation could be a safe and effective means of promoting chemotoxin delivery to cancer cells, potentially reducing the dosages and side effects of anti-cancer drugs.
将MCF - 7乳腺癌细胞和PC - 3前列腺癌细胞暴露于2.45 GHz的10 W微波下，可使它们从培养基中摄取抗癌药物阿霉素的量增加近100%，同时增加细胞死亡，而单独的微波照射没有细胞毒性。在用阿霉素和微波处理MCF - 7细胞期间添加内吞作用抑制剂，对抗癌药物的摄取没有影响。此外，MCF - 7细胞对寡核苷酸的摄取不受微波处理的影响。这些观察结果表明，在细胞暴露于微波辐射时，内吞作用不参与阿霉素的摄取。因此，靶向低功率微波辐射可能是一种安全有效的方法，可促进化学毒素传递到癌细胞，有可能降低抗癌药物的剂量和副作用。
