Tang Xiaoxuan, Gu Xinyi, Huang Tingting, Chen Xiaoli, Zhou Zhihao, Yang Yumin, Ling Jue
Key Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong 226001, PR China.
Medical School of Nantong University, Nantong University, Nantong 226001, PR China.
ACS Macro Lett. 2021 Dec 21;10(12):1501-1509. doi: 10.1021/acsmacrolett.1c00533. Epub 2021 Nov 15.
A lack of effective bioactivity to create a desirable microenvironment for peripheral nerve regeneration has been challenging in successful treatment of long-distance injuries using nerve guidance conduits (NGCs) clinically. Herein, we developed a silk-inspired phototriggered gelation system combining dual therapeutic cues of anisotropic topography and adhesive ligands for improving peripheral nerve regeneration. Importantly, enhanced cell recruitment and myelination of Schwann cells were successfully achieved by the Arg-Gly-Asp (RGD)-peptide-immobilized hydrogel scaffolds to promote axon growth. Moreover, as the orientated growth of Schwann cells and rapid axon growth were facilitated by aligned grooved micropatterns, this multifunctional bioactive system provides remarkable nerve regeneration with function recovery for long-distance nerve injury. Therefore, this bioengineered silk-inspired nerve guidance conduit delivers a platform for desirable peripheral nerve repair.
在临床上使用神经引导导管（NGC）成功治疗长距离损伤时，缺乏有效的生物活性以创建有利于周围神经再生的微环境一直是一项挑战。在此，我们开发了一种受丝绸启发的光触发凝胶化系统，该系统结合了各向异性拓扑结构和粘附配体的双重治疗线索，以促进周围神经再生。重要的是，通过固定有精氨酸-甘氨酸-天冬氨酸（RGD）肽的水凝胶支架成功实现了增强的细胞募集和雪旺细胞的髓鞘形成，从而促进轴突生长。此外，由于对齐的带槽微图案促进了雪旺细胞的定向生长和轴突的快速生长，这种多功能生物活性系统为长距离神经损伤提供了具有功能恢复的显著神经再生。因此，这种生物工程的受丝绸启发的神经引导导管为理想的周围神经修复提供了一个平台。
