一种用于测定小鼠血清中溶血补体活性的微量测定法。

A microassay for the determination of hemolytic complement activity in mouse serum.

作者信息

Andrews B S, Theofilopoulos A N

出版信息

J Immunol Methods. 1978;22(3-4):273-81. doi: 10.1016/0022-1759(78)90035-2.

Abstract

A 51Cr release microhemolytic complement assay is described to detect hemolytic complement activity in mouse serum, 51Cr-labeled sheep erythrocytes (SRBC) which have been sensitized with a subagglutinating amount of the 7S IgG fraction of rabbit anti-SRBC serum are placed in microtiter plates and further antibody added to each well prior to the addition of the complement (C) source. The IgG antibody was found to be more efficient in the lytic assay than the 19S IgM antibody. The assay is simple to perform, reproducible, and requires small volumes of mouse serum. Comparative hemolytic values were established for serum from a variety of murine strains using pooled BALB/c serum as the C reference. No apparent relationship was noted between the H-2 type of inbred mouse strains and the presence or absence of hemolytic complement.

摘要

描述了一种51Cr释放微溶血补体测定法，用于检测小鼠血清中的溶血补体活性。将用亚凝集量的兔抗绵羊红细胞（SRBC）血清的7S IgG组分致敏的51Cr标记的绵羊红细胞（SRBC）置于微量滴定板中，并在加入补体（C）来源之前向每个孔中加入进一步的抗体。发现IgG抗体在溶血测定中比19S IgM抗体更有效。该测定法操作简单、可重复，并且需要少量的小鼠血清。以汇集的BALB/c血清作为C参考，建立了各种小鼠品系血清的比较溶血值。在近交小鼠品系的H-2类型与溶血补体的存在与否之间未观察到明显关系。