Sasaki T, Ishida S, Onodera S, Saito T, Furuyama T, Yoshinaga K
J Immunol Methods. 1978;22(3-4):327-37. doi: 10.1016/0022-1759(78)90040-6.
Passive hemagglutination (PHA) and hemolysis (PHL) tests using chromium chloride-treated sheep red blood cells were developed to detect and measure the anti-DNA antibodies. Sonication of native DNA was found to prevent the incidence of non-specific agglutination. Sheep red cells were coated with double-stranded DNA (dsDNA) which had been sonicated and treated with nuclease S1 to digest the single-stranded regions in the DNA. The specificities for dsDNA-coated cells were checked by inhibition studies in PHA test and plaque assay. In clinical studies fairly close correlations were found between the antibodies to DNA and the activity of the disease in patients with systemic lupus erythematosus (SLE). Complement-fixing antibodies were detected in most of SLE patients with active lupus nephritis, but rarely in those in remission. Anticomplementary activity seemed to be negligible in PHL test. These tests are simple and may be useful to the diagnosis and the management of SLE.
利用经氯化铬处理的绵羊红细胞开展了被动血凝试验（PHA）和溶血试验（PHL），以检测和测定抗DNA抗体。研究发现，天然DNA的超声处理可防止非特异性凝集的发生。用经超声处理并用核酸酶S1处理以消化DNA中单链区域的双链DNA（dsDNA）包被绵羊红细胞。通过PHA试验和噬斑测定中的抑制研究来检查dsDNA包被细胞的特异性。在临床研究中，发现系统性红斑狼疮（SLE）患者的DNA抗体与疾病活动度之间存在相当密切的相关性。在大多数活动性狼疮性肾炎的SLE患者中检测到补体结合抗体，但在缓解期患者中很少检测到。在PHL试验中，抗补体活性似乎可以忽略不计。这些试验操作简单，可能对SLE的诊断和管理有用。
