Starosta Ewa, Szwarc Justyna, Niemann Janetta, Szewczyk Katarzyna, Weigt Dorota
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland.
Curr Issues Mol Biol. 2023 May 18;45(5):4431-4450. doi: 10.3390/cimb45050282.
Rapeseed is one of the most important oil crops in the world. Increasing demand for oil and limited agronomic capabilities of present-day rapeseed result in the need for rapid development of new, superior cultivars. Double haploid (DH) technology is a fast and convenient approach in plant breeding as well as genetic research. is considered a model species for DH production based on microspore embryogenesis; however, the molecular mechanisms underlying microspore reprogramming are still vague. It is known that morphological changes are accompanied by gene and protein expression patterns, alongside carbohydrate and lipid metabolism. Novel, more efficient methods for DH rapeseed production have been reported. This review covers new findings and advances in DH production as well as the latest reports related to agronomically important traits in molecular studies employing the double haploid rapeseed lines.
油菜是世界上最重要的油料作物之一。对油脂需求的不断增加以及当今油菜有限的农艺性能,导致需要快速培育新的优良品种。双单倍体(DH)技术是植物育种和遗传研究中一种快速便捷的方法。基于小孢子胚胎发生,油菜被认为是DH生产的模式物种;然而,小孢子重编程的分子机制仍不明确。已知形态变化伴随着基因和蛋白质表达模式以及碳水化合物和脂质代谢。已报道了用于DH油菜生产的新颖、更有效的方法。本综述涵盖了油菜DH生产的新发现和进展,以及利用双单倍体油菜品系进行分子研究中与农艺重要性状相关的最新报道。