Wirtz R A, Zavala F, Charoenvit Y, Campbell G H, Burkot T R, Schneider I, Esser K M, Beaudoin R L, Andre R G
Bull World Health Organ. 1987;65(1):39-45.
Ten monoclonal antibodies developed against Plasmodium falciparum sporozoites at four institutions were evaluated for use in an enzyme-linked immunosorbent assay (ELISA). Four of the antibodies were eliminated because of their low sensitivity or requirement for high concentrations of capture antibody, while an additional four were rejected because they exhibited cross-reactivity with P. berghei sporozoites. Of the two remaining monoclonal antibodies, that designated 2A10 had the highest sensitivity, a requirement for lower concentrations of capture antibody, and had been tested successfully against sporozoites from a wider range of geographical areas than the others. Use of this monoclonal antibody in a standardized ELISA method gave a test ten times more sensitive than previously reported for P. falciparum sporozoites and its detection limit was less than 100 sporozoites per mosquito.
对四个机构研发的针对恶性疟原虫子孢子的十种单克隆抗体进行了酶联免疫吸附测定（ELISA）适用性评估。四种抗体因灵敏度低或对捕获抗体浓度要求高而被淘汰，另有四种因与伯氏疟原虫子孢子表现出交叉反应性而被排除。剩下的两种单克隆抗体中，命名为2A10的抗体灵敏度最高，对捕获抗体浓度要求较低，并且与其他抗体相比，已成功针对更广泛地理区域的子孢子进行了测试。在标准化ELISA方法中使用这种单克隆抗体，检测恶性疟原虫的灵敏度比之前报道的高十倍，其检测限为每只蚊子少于100个子孢子。
