Fahmi Mochamad Zakki, Haris Abdul, Permana Ahmadi Jaya, Nor Wibowo Denika Liyan, Purwanto Bambang, Nikmah Yatim Lailun, Idris Adi
Department of Chemistry, Universitas Airlangga Surabaya 61115 Indonesia
Department of Physiology, Department of Medical Biochemistry, Faculty of Medicine, Universitas Airlangga Surabaya 60131 Indonesia.
RSC Adv. 2018 Nov 14;8(67):38376-38383. doi: 10.1039/c8ra07944g.
In this study, carbon dots synthesized from bamboo leaf cellulose were used simultaneously as a staining agent and for doxorubicin delivery to target cancer cells. Owing to their nontoxic properties, the production of carbon dots from bamboo leaves is a green approach involving optimized application of bamboo tree waste. For multifunctional applications, the carbon dots were modified with 4-carboxybenzylboronic acid and doxorubicin to improve target specificity and drug delivery to HeLa tumor cells. The resulting modified carbon dots were characterized using different analytical techniques, which showed that they were biocompatible, nontoxic, and highly stable over a wide range of pH values and at high ionic strengths. Furthermore, confocal microscopy studies demonstrated their blue fluorescence and cellular pathway for entering HeLa cells folate receptor-mediated endocytosis. Cell viability data and flow cytometry results also confirmed the selective uptake of the carbon dots by HeLa cells, which significantly enhanced cell cytotoxicity.
在本研究中，由竹叶纤维素合成的碳点被同时用作染色剂和用于将阿霉素递送至靶癌细胞。由于其无毒特性，从竹叶中生产碳点是一种绿色方法，涉及对竹树废料的优化利用。为了实现多功能应用，用4-羧基苄基硼酸和阿霉素对碳点进行了修饰，以提高对HeLa肿瘤细胞的靶向特异性和药物递送能力。使用不同的分析技术对所得的修饰碳点进行了表征，结果表明它们具有生物相容性、无毒，并且在很宽的pH值范围和高离子强度下都高度稳定。此外，共聚焦显微镜研究证明了它们的蓝色荧光以及通过叶酸受体介导的内吞作用进入HeLa细胞的细胞途径。细胞活力数据和流式细胞术结果也证实了HeLa细胞对碳点的选择性摄取，这显著增强了细胞毒性。
