Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, East Java, Indonesia.
Biotechnology of Tropical Medicinal Plants Research Group, Universitas Airlangga, Surabaya, East Java, Indonesia.
Int J Nanomedicine. 2023 Aug 3;18:4471-4484. doi: 10.2147/IJN.S403385. eCollection 2023.
Breast cancer is one of the main causes of death in women. is an Indonesian herbal plant that can be used as an anti-cancer. However, herbal medicines have low bioavailability, which affects their bioactivity. Nanoencapsulation can increase bioavailability and stability of bioactive compounds in herbal medicines.
This recent finding tried to unravel anti-cancer and chemopreventive of nano-encapsulated by Na-alginate.
bioactive compounds were isolated and characterized using UV-Vis spectrometer, FTIR, NMR and HR-MS. extract was nanoencapsulated using Na-alginate. Anti-cancer effect was assessed by MTT assay towards T47D cell. Meanwhile, a chemopreventive analysis was carried out in breast cancer mice-induced benzo[α]pyrene. The healthy mice were divided into 8 groups comprising control and treatment.
Elucidation of ethyl acetate extract confirmed high catechin content, 89.34% (w/w). Successful nanoencapsulation of () was indicated. The particle size of was 78.40 ± 12.25 nm. Loading efficiency (LE) and loading amount (LA) of were 97.56 ± 0.04% and 32.52 ± 0.01%, respectively. had an EC value of 10.39 ± 3.50 µg/mL, which was more toxic than the EC value of extract towards the T47D cell line. Administration of 200 mg/kg BW to mice induced by benzo[α]pyrene exhibited SOD and GSH levels of 13.69 ng/mL and 455.6 ng/mL. In addition, the lowest TNF-α level was 27.96 ng/mL. A dose of 100 mg/kg BW could best increase CAT levels by 7.18 ng/mL. There was no damage or histological abnormalities found in histological analysis of the breast tissue in the group given 200 mg/kg BW .
乳腺癌是导致女性死亡的主要原因之一。是一种印度尼西亚草药植物，可用作抗癌药物。然而，草药的生物利用度低，这影响了它们的生物活性。纳米胶囊化可以提高草药中生物活性化合物的生物利用度和稳定性。
本研究试图揭示 Na-海藻酸钠纳米胶囊化对 的抗癌和化学预防作用。
使用紫外可见分光光度计、傅里叶变换红外光谱仪、核磁共振和高分辨质谱对生物活性化合物进行分离和表征。使用 Na-海藻酸钠对 提取物进行纳米胶囊化。通过 MTT 法测定 T47D 细胞对 的抗癌作用。同时，在苯并[α]芘诱导的乳腺癌小鼠中进行化学预防分析。健康小鼠分为 8 组，包括对照组和治疗组。
乙酸乙酯提取物的解析证实儿茶素含量高，为 89.34%（w/w）。成功地对 （）进行了纳米胶囊化。 的粒径为 78.40 ± 12.25 nm。 的载药量（LE）和载药量（LA）分别为 97.56 ± 0.04%和 32.52 ± 0.01%。 的 EC 值为 10.39 ± 3.50 µg/mL，比提取物对 T47D 细胞系的 EC 值更具毒性。给予苯并[α]芘诱导的小鼠 200 mg/kg BW 可使 SOD 和 GSH 水平分别达到 13.69 ng/mL 和 455.6 ng/mL。此外，TNF-α 水平最低为 27.96 ng/mL。100 mg/kg BW 的剂量可使 CAT 水平最佳增加 7.18 ng/mL。在给予 200 mg/kg BW 的组中，在乳房组织的组织学分析中未发现任何损伤或组织学异常。
