Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja 719-1197, Okayama, Japan.
Graduate School of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja 719-1197, Okayama, Japan.
Int J Mol Sci. 2022 Apr 23;23(9):4691. doi: 10.3390/ijms23094691.
Mitochondrial function in skeletal muscle, which plays an essential role in oxidative capacity and physical activity, declines with aging. Acetic acid activates AMP-activated protein kinase (AMPK), which plays a key role in the regulation of whole-body energy by phosphorylating key metabolic enzymes in both biosynthetic and oxidative pathways and stimulates gene expression associated with slow-twitch fibers and mitochondria in skeletal muscle cells. In this study, we investigate whether long-term supplementation with acetic acid improves age-related changes in the skeletal muscle of aging rats in association with the activation of AMPK. Male Sprague Dawley (SD) rats were administered acetic acid orally from 37 to 56 weeks of age. Long-term supplementation with acetic acid decreased the expression of atrophy-related genes, such as atrogin-1, muscle RING-finger protein-1 (MuRF1), and transforming growth factor beta (TGF-β), activated AMPK, and affected the proliferation of mitochondria and type I fiber-related molecules in muscles. The findings suggest that acetic acid exhibits an anti-aging function in the skeletal muscles of aging rats.
线粒体在骨骼肌中的功能对于氧化能力和身体活动至关重要，随着年龄的增长而下降。乙酸激活 AMP 激活的蛋白激酶（AMPK），它通过磷酸化生物合成和氧化途径中的关键代谢酶，以及刺激与骨骼肌慢肌纤维和线粒体相关的基因表达，在调节全身能量方面发挥着关键作用。在这项研究中，我们研究了长期补充乙酸是否可以改善与 AMPK 激活相关的衰老大鼠骨骼肌的衰老变化。雄性 Sprague Dawley（SD）大鼠从 37 周到 56 周龄时口服给予乙酸。长期补充乙酸可降低萎缩相关基因的表达，如 atrogin-1、肌肉环指蛋白 1（MuRF1）和转化生长因子-β（TGF-β），激活 AMPK，并影响肌肉中线粒体和 I 型纤维相关分子的增殖。这些发现表明，乙酸在衰老大鼠的骨骼肌中表现出抗衰老功能。
