Yuan Qin, Zhou Qianqian, Wang Nanlan, Zheng Yuancui, Hu Hua, Hu Shiyao, Wang Huiqun
School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China.
Guizhou Food Nutrition and Health Engineering Research Center, Guiyang, China.
Front Nutr. 2022 Nov 3;9:1019205. doi: 10.3389/fnut.2022.1019205. eCollection 2022.
Miao Sour Soup (MSS) is a fermented product from the Qiandongnan region of Guizhou Province, which enrich many beneficial ingredients and is widely consumed in the whole China. Fermented food is beneficial to physical health with the potential positive regulating affection on simple obesity. In this study, we analyzed the mechanism of action of MSS to prevent simple obesity induced by high-fat diet by proteomics and metabolomics. Quantitative proteomics with tandem mass tagging labeling and liquid chromatography-mass spectrometry was used to analyze the changes of liver proteins and metabolites after the MSS intervention. MSS intervention upregulated 33 proteins and 9 metabolites and downregulated 19 proteins and 10 metabolites. Bioinformatics analysis showed that MSS could prevent simple obesity by acting on the PPAR signaling pathway, retinol metabolism, fatty acid β-oxidation, fatty acid degradation, fatty acid biosynthesis, glycine, serine and threonine metabolism, pyruvate metabolism, citrate cycle (TCA cycle) and other signaling pathways. This study provides new insights into the use of MSS to prevent simple obesity caused by high-fat diets and the search for healthy eating patterns with MSS.
苗家酸汤（MSS）是贵州省黔东南地区的一种发酵食品，富含多种有益成分，在全中国广泛食用。发酵食品对身体健康有益，对单纯性肥胖具有潜在的正向调节作用。在本研究中，我们通过蛋白质组学和代谢组学分析了MSS预防高脂饮食诱导的单纯性肥胖的作用机制。采用串联质量标签标记和液相色谱-质谱联用的定量蛋白质组学方法分析MSS干预后肝脏蛋白质和代谢物的变化。MSS干预使33种蛋白质和9种代谢物上调，19种蛋白质和10种代谢物下调。生物信息学分析表明，MSS可通过作用于过氧化物酶体增殖物激活受体（PPAR）信号通路、视黄醇代谢、脂肪酸β-氧化、脂肪酸降解、脂肪酸生物合成、甘氨酸、丝氨酸和苏氨酸代谢、丙酮酸代谢、柠檬酸循环（TCA循环）等信号通路来预防单纯性肥胖。本研究为利用MSS预防高脂饮食引起的单纯性肥胖以及探索以MSS为特色的健康饮食模式提供了新的见解。
