Strawberry Regulates Anaerobic Respiratory Metabolism under Waterlogging.

Sucrose nonfermenting-1-related protein kinase 1 (SnRK1) is a central integrator of plant stress and energy starvation signalling pathways. We found that the -overexpression (OE) roots had a higher respiratory rate and tolerance to waterlogging than the -RNAi roots, suggesting that plays a positive role in the regulation of anaerobic respiration under waterlogging. upregulated the activity of anaerobic respiration-related enzymes including hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH). also enhanced the ability to quench reactive oxygen species (ROS) by increasing antioxidant enzyme activities. We sequenced the transcriptomes of the roots of both wild-type (WT) and -RNAi plants, and the differentially expressed genes (DEGs) were clearly enriched in the defence response, response to biotic stimuli, and cellular carbohydrate metabolic process. In addition, 42 genes involved in glycolysis and 30 genes involved in pyruvate metabolism were significantly regulated in -RNAi roots. We analysed the transcript levels of two anoxia-related genes and three , and the results showed that , , and were upregulated in response to , indicating that may be involved in the ethylene signalling pathway to improve waterlogging tolerance. In conclusion, increases the expression of and further activates anoxia response genes, thereby enhancing anaerobic respiration metabolism in response to low-oxygen conditions during waterlogging.