基于雌激素受体鸡尾酒法的乳制品中雌激素干扰化合物筛查
Screening of Estrogenic-Disrupting Compounds in Dairy Products Based on the Estrogen Receptor Cocktail.
作者信息
Li Xiaoqi, Du Shuyuan, Tian Fangyuan, Wang Minglu, Wang Ying, Zhang Hongyan, Zang Liguo
机构信息
Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, China.
College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
出版信息
Foods. 2022 Apr 19;11(9):1178. doi: 10.3390/foods11091178.
The residue of estrogenic-disrupting compounds (EDCs) that are secreted by cows, added as drugs, and present in the feed may exist in dairy products. A gold nanoparticles (AuNPs)-estrogen receptor (ER) cocktail colorimetric assay equipped with ER cocktail solid phase extraction (SPE) was established to screen EDCs. Nine EDCs with high, moderate, and low estrogenic activity were selected to be the representative targets. The recognition range of the colorimetric assay combined with the ER cocktail SPE was wider than that of a single ERα or ERβ. The lowest detection limit of the established assay was about 10 mg·mL. The detection limits of estrone, bisphenol A, and bisphenol B were about one order of magnitude lower than the method based on a single ER. The recoveries of the spiked nine EDCs were between 80.0% and 110.0%, and daidzein was identified in the dairy product. The developed method has potential application prospects in food safety and environmental monitoring.
奶牛分泌的、作为药物添加的以及存在于饲料中的雌激素干扰化合物（EDCs）残留物可能存在于乳制品中。建立了一种配备雌激素受体（ER）鸡尾酒固相萃取（SPE）的金纳米颗粒（AuNPs）-雌激素受体（ER）比色测定法来筛选EDCs。选择了九种具有高、中、低雌激素活性的EDCs作为代表性目标物。结合ER鸡尾酒SPE的比色测定法的识别范围比单一的ERα或ERβ更广。所建立测定法的最低检测限约为10 mg·mL。雌酮、双酚A和双酚B的检测限比基于单一ER的方法低约一个数量级。加标的九种EDCs的回收率在80.0%至110.0%之间，并且在乳制品中鉴定出了大豆苷元。所开发的方法在食品安全和环境监测方面具有潜在的应用前景。
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