采用酶联免疫吸附测定法检测新西兰人血清中的破伤风类毒素抗体。
Detection of tetanus toxoid antibodies in human sera in New Zealand by ELISA.
作者信息
Lau R C
出版信息
Epidemiol Infect. 1987 Apr;98(2):199-202. doi: 10.1017/s0950268800061914.
Abstract
An enzyme-linked immunosorbent assay (ELISA) incorporating the sensitive biotin-streptavidin system was developed to detect IgG antibodies to tetanus toxoid in human serum. Serum samples obtained from 557 normal persons aged 1-65 years from different areas in New Zealand were tested. The proportion of those immune ranged from 60-93% in males, and from 46-86% in females. In the 1-9 years age group 85% were immune. The indirect ELISA is suitable for serological surveys as it is simple to perform, economical and reproducible.
摘要
开发了一种结合敏感生物素-链霉亲和素系统的酶联免疫吸附测定（ELISA），用于检测人血清中破伤风类毒素的IgG抗体。对从新西兰不同地区557名年龄在1至65岁的正常人获得的血清样本进行了检测。免疫者的比例在男性中为60%-93%，在女性中为46%-86%。在1至9岁年龄组中，85%的人具有免疫力。间接ELISA操作简单、经济且可重复，适用于血清学调查。
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