D. Gary Young Research Institute, Lehi, UT 84043, USA.
Molecules. 2022 Apr 27;27(9):2777. doi: 10.3390/molecules27092777.
Mill. (Peruvian basil) is an essential oil-bearing plant of the Lamiaceae family. Volatile oil produced through steam distillation of Peruvian basil was examined to establish the aromatic and stable isotope profiles of samples ( = 9) from three different cultivated plots in Peru. The resulting essential oils were analyzed by GC/FID, GC/MS, and GC/IRMS. In accordance with findings from other researchers, multiple chemotypes, defined by the most abundant aromatic compounds, exist within these populations. Overall, 55% of samples are the eugenol chemotype (values ranging 15.4-30.2%), 33% are the methyl eugenol chemotype (values ranging 68.1-68.7%), and a single sample is a mixture of both chemotypes, containing high levels of both eugenol (38.1%) and methyl eugenol (8.6%). Stable isotope ratios, H and C, performed on prominent compounds provide supporting data for distinguishing chemotypes. Complete aromatic profiles, stable isotope ratios, and essential oil yield are established for each sample. This study confirms the existence of multiple chemotypes and, for the first time, to the author's best knowledge, establishes stable isotope ratios for essential oil, which proves a useful tool in further investigating plant metabolism and determining essential oil authenticity.
罗勒（秘鲁）是唇形科的一种含油植物。通过对秘鲁罗勒的水蒸气蒸馏产生的挥发性油进行了研究，以确定来自秘鲁三个不同种植区的 9 个样本的芳香族和稳定同位素分布。用 GC/FID、GC/MS 和 GC/IRMS 对得到的精油进行了分析。与其他研究人员的发现一致，这些种群中存在多种化学型，由最丰富的芳香族化合物定义。总体而言，55%的样品属于丁香酚化学型（数值范围为 15.4-30.2%），33%属于甲基丁香酚化学型（数值范围为 68.1-68.7%），只有一个样品是两种化学型的混合物，含有高浓度的丁香酚（38.1%）和甲基丁香酚（8.6%）。对显著化合物进行的 H 和 C 稳定同位素比值提供了区分化学型的辅助数据。为每个样品建立了完整的芳香族图谱、稳定同位素比值和精油产量。本研究证实了多种化学型的存在，并且据作者所知，首次为精油建立了稳定同位素比值，这是进一步研究植物代谢和确定精油真实性的有用工具。
