Department of Cell and Chemical Biology and Oncode Institute, Leiden University Medical Center, Leiden, The Netherlands; Department of Chemical Engineering, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Department of Chemical Engineering, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Trends Cancer. 2022 Aug;8(8):683-697. doi: 10.1016/j.trecan.2022.03.006. Epub 2022 May 12.
An early step of metastasis requires a complex and coordinated migration of invasive tumor cells into the surrounding tumor microenvironment (TME), which contains extracellular matrix (ECM). It is being appreciated that 3D matrix-based microfluidic models have an advantage over conventional in vitro and animal models to study tumor progression events. Recent microfluidic models have enabled recapitulation of key mechanobiological features present within the TME to investigate collective cancer cell migration and invasion. Microfluidics also allows for functional interrogation and therapeutic manipulation of specific steps to study the dynamic aspects of tumor progression. In this review, we focus on recent developments in cancer cell migration and how microfluidic strategies have evolved to address the physiological complexities of the TME to visualize migration modes adapted by various tumor cells.
转移的早期步骤需要侵袭性肿瘤细胞复杂而协调地迁移到周围的肿瘤微环境（TME）中，其中包含细胞外基质（ECM）。人们越来越认识到，基于 3D 基质的微流控模型在研究肿瘤进展事件方面优于传统的体外和动物模型。最近的微流控模型使人们能够再现 TME 中存在的关键机械生物学特征，以研究癌细胞的集体迁移和侵袭。微流控还允许对特定步骤进行功能检测和治疗操作，以研究肿瘤进展的动态方面。在这篇综述中，我们重点介绍了癌细胞迁移的最新进展，以及微流控策略如何发展以应对 TME 的生理复杂性，从而可视化各种肿瘤细胞所采用的迁移模式。
