Key Laboratory of Textile Fabrics, College of Textiles and Clothing, Anhui Polytechnic University, Wuhu, Anhui 241000, PR China; Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Bioresour Technol. 2022 Jul;356:127311. doi: 10.1016/j.biortech.2022.127311. Epub 2022 May 12.
Laccase is a promising biocatalyst for pollutant degradation and water purification. However, laccase can only improve the stability of enzyme activity and achieve its significant catalytic effect after effective immobilization. Herein, we report a general strategy to integrate nanocellulose aerogel and laccase for high-efficiency degradation of organic pollutants. Biomass-derived functional bacterial cellulose (BC) aerogel with a nanonetwork structure and high porosity was prepared by biosynthesis, solvent replacement, and atom transfer radical polymerization (ATRP) procedures. Subsequently, a biocatalyst platform was fabricated by "coupling" ATRP-modified BC aerogel with abundant active sites with laccase through ion coordination. The results demonstrated the biocatalyst platform not only has good biological affinity, but also has high enzyme load and structural stability. Meanwhile, the degradation rates of reactive red X-3B and 2, 4-dichlorophenol reached 94.5% and 85.2% within 4 h, respectively. The strategy disclosed herein could provide a practical method for the degradation of organic pollutants.
漆酶是一种很有前途的生物催化剂，可用于污染物降解和水净化。然而，只有经过有效的固定化，漆酶才能提高酶活性的稳定性并发挥其显著的催化效果。在此，我们报告了一种将纳米纤维素气凝胶与漆酶集成的通用策略，用于高效降解有机污染物。通过生物合成、溶剂置换和原子转移自由基聚合（ATRP）程序，制备了具有纳米网络结构和高孔隙率的生物质衍生功能细菌纤维素（BC）气凝胶。随后，通过离子配位将具有丰富活性位点的 ATRP 修饰的 BC 气凝胶与漆酶“偶联”，构建了生物催化剂平台。结果表明，该生物催化剂平台不仅具有良好的生物亲和性，而且具有较高的酶载量和结构稳定性。同时，反应性红 X-3B 和 2,4-二氯苯酚在 4 h 内的降解率分别达到 94.5%和 85.2%。本文所揭示的策略为有机污染物的降解提供了一种实用的方法。
