Department of Interventional Neuroradiology, Beijing Neurosurgical Institute and Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, PR China.
School of Pharmaceutical Sciences, Capital Medical University, Beijing, 100069, PR China.
Biochem Biophys Res Commun. 2022 Jul 23;614:125-131. doi: 10.1016/j.bbrc.2022.04.143. Epub 2022 May 5.
Timely and accurate diagnosis of acute ischemic stroke (AIS) and simultaneous functional imaging of cerebral oxygen saturation (sO) are essential to improve the survival rate of stroke patients but remains challenging. Herein, we developed a pH-responsive manganese (Mn)-based nanoplatform as a magnetic resonance/photoacoustic (MR/PA) dual-modal contrast agent for AIS diagnosis. The Mn-based nanoplatform was prepared via a simple and green biomimetic method using bovine serum albumin (BSA) as a scaffold for fabrication of MnCO NPs as the T MR contrast agent and accommodation of indocyanine green (ICG) as the PA probe. The obtained MnCO@BSA-ICG NPs were biocompatible and exhibited a pH-responsive longitudinal relaxation rate and a concentration-dependent PA signal. In vivo MR/PA dual-modal imaging demonstrated that MnCO@BSA-ICG NPs quickly and efficiently led to the MR/PA contrast enhancements in the infarcted area while not in the normal region, allowing a timely and accurate diagnosis of AIS. Moreover, PA imaging could directly monitor the sO level, enabling a functional imaging of AIS. Therefore, MnCO@BSA-ICG NPs could be applied as a potential MR/PA contrast agent for timely and functional imaging of AIS.
及时准确地诊断急性缺血性脑卒中（AIS）并同时对脑氧饱和度进行功能成像对于提高脑卒中患者的生存率至关重要，但目前仍具有挑战性。在此，我们开发了一种 pH 响应性的锰（Mn）基纳米平台，作为磁共振/光声（MR/PA）双模式对比剂，用于 AIS 的诊断。该 Mn 基纳米平台是通过一种简单而绿色的仿生方法制备的，使用牛血清白蛋白（BSA）作为支架，用于制造 MnCO NPs 作为 T1 MR 对比剂，并容纳吲哚菁绿（ICG）作为 PA 探针。所得的 MnCO@BSA-ICG NPs 具有良好的生物相容性，表现出 pH 响应性的纵向弛豫率和浓度依赖性的 PA 信号。体内 MR/PA 双模式成像表明，MnCO@BSA-ICG NPs 能够快速有效地导致梗死区域的 MR/PA 对比度增强，而在正常区域则没有，从而能够及时准确地诊断 AIS。此外，PA 成像可以直接监测 sO 水平，实现 AIS 的功能成像。因此，MnCO@BSA-ICG NPs 可用作潜在的 MR/PA 对比剂，用于 AIS 的及时和功能成像。
