Centre of Research and Water Technologies (CERTE), Laboratory of Treatment and Valorisation of Water Rejects (LTVRH), Techno Park of Borj-Cédria, 8020, Borj Cédria, Tunisia.
Faculty of Mathematical, Physical and Natural Sciences of Tunis, University of Tunis El Manar, 2092, Tunis, Tunisia.
Food Environ Virol. 2020 Sep;12(3):250-259. doi: 10.1007/s12560-020-09434-0. Epub 2020 Jul 1.
The molecular detection of Norovirus GI and Norovirus GII in the Tunisian industrial wastewater treatment plant of Charguia I was conducted to test the effectiveness of secondary biological treatment using the activated sludge procedure and the UV-C tertiary treatment radiation using a UV disinfection prototype to upgrade the quality of the purified wastewater. A total of 140 sewage samples were collected from the two lines of sewage treatment procedures. Norovirus GI and Norovirus GII have been found and quantified using Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) in 66.4 and 86.4% of the collected wastewater samples. The Norovirus GI and GII mean concentration values got in the treated effluents showed a significant decrease of Norovirus viral content rates detected from upstream to downstream of activated sludge procedures and at the output of UV-C treatment. These findings characterise and denote for the first time the effectiveness of biological and UV-C254 treatment for Norovirus GI and Norovirus GII removal in Tunis City, northern Tunisia. Also, these data underlined the fact that purified sewage makes up a route of gastroenteritis virus transmission and a cause of viral gastroenteritis associated with water-borne and food-borne outbreaks.
对突尼斯 Charguia I 地区工业污水处理厂中的诺如病毒 GI 和诺如病毒 GII 进行了分子检测，以检验采用活性污泥工艺的二级生物处理和使用紫外线-C 三级处理辐射（使用紫外线消毒原型）来提高净化后污水质量的效果。从两条污水处理线共采集了 140 个污水样本。使用实时逆转录聚合酶链反应（qRT-PCR）发现并定量了 66.4%和 86.4%的采集污水样本中存在的诺如病毒 GI 和诺如病毒 GII。在处理后的废水中获得的诺如病毒 GI 和 GII 的平均浓度值表明，在活性污泥工艺的上下游以及紫外线-C 处理的出口处，诺如病毒病毒含量的检测率显著下降。这些发现首次对突尼斯北部突尼斯市的生物和紫外线-C254 处理去除诺如病毒 GI 和诺如病毒 GII 的效果进行了描述和说明。此外，这些数据强调了净化后的污水是肠胃炎病毒传播的途径，也是与水传播和食源性疾病暴发相关的病毒性肠胃炎的一个原因。
