Chamandy Abir, Zhao Minjie, Rammal Hassan, Ennahar Saïd
Analytical Chemistry of Bioactive Molecules and Pharmacognosy Team (CAMBAP), Hubert Curien Multidisciplinary Institute (IPHC), National Center for Scientific Research (CNRS), Faculty of Pharmacy, University of Strasbourg, Illkirch, 67400, France.
Research Platform of Analysis and Environmental Science (PRASE), Lebanese University, Hadath, 005, Lebanon.
J Pharm Anal. 2022 Apr;12(2):253-262. doi: 10.1016/j.jpha.2021.09.008. Epub 2021 Sep 16.
The compounds in leaf and stem extracts of Labill. (AEL), a plant species used in traditional Lebanese medicine, were investigated for antioxidant properties. First, the activity of various extracts was assessed using the Trolox equivalent antioxidant capacity, oxygen radical absorption capacity, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate assays. The extract obtained using 30% ethanol showed the greatest activity. The antioxidant compounds in this extract were screened using a hyphenated high-performance liquid chromatography-2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) radical (ABTS) system before being separated by ultra-high-performance liquid chromatography and identified using high-resolution mass spectrometry and ultra-violet-visible diode array detection. Approximately 40 compounds were identified. Hydroxycinnamates (caffeic, ferulic, and -coumaric acid derivatives) and flavonoids (quercetin, luteolin, apigenin, and isorhamnetin derivatives) were the two main categories of the identified compounds. The active compounds were identified as caffeic acid derivatives and quercetin glycosides. In addition, the catechol moiety was shown to be key to antioxidant activity. This study showed that AEL is a source of natural antioxidants, which may explain its medicinal use.
对黎巴嫩传统医学中使用的一种植物——拉比勒（Labill.）叶和茎提取物中的化合物进行了抗氧化性能研究。首先，使用特洛克斯等效抗氧化能力、氧自由基吸收能力和2,2-二苯基-1-苦基肼基水合物测定法评估了各种提取物的活性。使用30%乙醇获得的提取物显示出最大活性。在通过超高效液相色谱分离并用高分辨率质谱和紫外可见二极管阵列检测进行鉴定之前，使用联用高效液相色谱-2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)自由基（ABTS）系统对该提取物中的抗氧化化合物进行了筛选。鉴定出了约40种化合物。羟基肉桂酸（咖啡酸、阿魏酸和对香豆酸衍生物）和黄酮类化合物（槲皮素、木犀草素、芹菜素和异鼠李素衍生物）是鉴定出的化合物的两大类。活性化合物被鉴定为咖啡酸衍生物和槲皮素糖苷。此外，邻苯二酚部分被证明是抗氧化活性的关键。这项研究表明，拉比勒叶和茎提取物是天然抗氧化剂的一个来源，这可能解释了它的药用价值。
