Department of Biological and Chemical Engineering, Biocatalysis and Bioprocessing Group, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus, Denmark.
Institute of Technical Chemistry, Leibniz University Hannover, Callinstr. 5, 30167, Hannover, Germany.
Angew Chem Int Ed Engl. 2022 Aug 1;61(31):e202203823. doi: 10.1002/anie.202203823. Epub 2022 Jun 21.
The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catalyzed by horse liver alcohol dehydrogenase was performed in a larger-scale experiment, and the biocatalyst could be recycled by simply removing the DES phase for three recycling runs.
本研究考察了由疏水性深共晶溶剂（DES）和具有较低临界溶解温度（LCST）相转变的水相磷酸钾缓冲液组成的热致多相体系（TMS）在均相生物催化中的适用性。以脂肪油酸为氢键供体的基于利多卡因的 DES 被研究。在本研究中确定并呈现了相图。我们测试了溶剂体系的不同附加成分，观察到浊点约每浓度单位降低 0.026°C。分配研究表明，蛋白质在水缓冲相中明显分配（>95%），而疏水性底物和反应物则（>95%）在富含 DES 的层中积累。最后，在较大规模的实验中进行了由马肝醇脱氢酶催化的还原反应，通过简单地除去 DES 相，可以将生物催化剂回收三次。
